ArticleProceedings of the National Academy of Sciences of the United States of America2005
Establishing genetic transformation for comparative developmental studies in the crustacean Parhyale hawaiensis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
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- Targeting the cell membrane in established and emerging model organisms.bioRxiv : the preprint server for biology · 2025Article
- The white gene as a transgenesis marker for the cricket Gryllus bimaculatus.G3 (Bethesda, Md.) · 2024Article
- Validation of heat-inducibleiScience · 2024Article
- Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis.The Journal of experimental biology · 2024Article
- Cephalopod-omics: Emerging Fields and Technologies in Cephalopod Biology.Integrative and comparative biology · 2023Article
- Validation of a heat-induciblebioRxiv : the preprint server for biology · 2023Article
- A molecular toolkit for superorganisms.Trends in genetics : TIG · 2021Review
- Evaluating the probability of CRISPR-based gene drive contaminating another species.Evolutionary applications · 2020Article
- Experimental duplication of bilaterian body axes in spider embryos: Holm's organizer and self-regulation of embryonic fields.Development genes and evolution · 2020Review
- Delivery methods for CRISPR/Cas9 gene editing in crustaceans.Marine life science & technology · 2020Article
- Developing an integrated understanding of the evolution of arthropod segmentation using fossils and evo-devo.Proceedings. Biological sciences · 2019Article
- The amphipod crustacean Parhyale hawaiensis: An emerging comparative model of arthropod development, evolution, and regeneration.Wiley interdisciplinary reviews. Developmental biology · 2019Review
- Analysis of the genetically tractable crustacean Parhyale hawaiensis reveals the organisation of a sensory system for low-resolution vision.BMC biology · 2019Article
- The "amphi"-brains of amphipods: new insights from the neuroanatomy ofFrontiers in zoology · 2019Article
- Article
- The genome of the crustaceaneLife · 2016Article
- CRISPR/Cas9-Mediated Genome Editing and Mutagenesis ofG3 (Bethesda, Md.) · 2016Article
- Article
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2 authors.
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Abstract
The amphipod crustacean Parhyale hawaiensis has been put forward as an attractive organism for evolutionary developmental comparisons, and considerable effort is being invested in isolating developmental genes and studying their expression patterns in this species. The scope of these studies could be significantly expanded by establishing means for genetic manipulation that would enable direct studies of gene functions to be carried out in this species. Here, we report the use of the Minos transposable element for the genetic transformation of P. hawaiensis. Transformed amphipods can be obtained from approximately 30% of surviving individuals injected with both a Minos element carrying the 3xP3-DsRed fluorescent marker and with mRNA encoding the Minos transposase. Integral copies of the transposon are inserted into the host genome and are stably inherited through successive generations. We have used reporter constructs to identify a muscle-specific regulatory element from Parhyale, demonstrating that this transformation vector can be used to test the activity of cis-regulatory elements in this species. The relatively high efficiency of this transgenic methodology opens new opportunities for the direct study of cis-regulatory elements and gene functions in Parhyale, allowing functional studies to be carried out beyond previously established model systems in insects.
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