ArticlePlant physiology2005
Light-dark changes in cytosolic nitrate pools depend on nitrate reductase activity in Arabidopsis leaf cells.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 77 citations in OpenAlex.
- The Maize ZmbHLH118 Transcription Factor Regulates Vacuolar Nitrate Loading by the NOAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Genetically Encoded Fluorescent Biosensors Enable Noninvasive Real-Time Visualization of Nitrate Dynamics in Intact Living Plants.Biosensors · 2026Article
- Homeostasis of ammonium and nitrate in plants.Quantitative plant biology · 2026Review
- Chloride transport and homeostasis in plants.Quantitative plant biology · 2025Review
- Review
- Photosynthetic-Product-Dependent Activation of Plasma Membrane H+-ATPase and Nitrate Uptake in Arabidopsis Leaves.Plant & cell physiology · 2023Article
- Proton exchange by the vacuolar nitrate transporter CLCa is required for plant growth and nitrogen use efficiency.The Plant cell · 2023Article
- In vivo visualization of nitrate dynamics using a genetically encoded fluorescent biosensor.Science advances · 2022Article
- A win-win scenario for photosynthesis and the plasma membrane HFrontiers in plant science · 2022Review
- Whether do plant cells sense nitrate changes without a sensor?Frontiers in plant science · 2022Article
- Vacuolar nitrate efflux requires multiple functional redundant nitrate transporter inFrontiers in plant science · 2022Article
- The Physiological Implications ofAntioxidants (Basel, Switzerland) · 2020Review
- Dynamic measurement of cytosolic pH and [NOProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Novel Aspects of Nitrate Regulation inFrontiers in plant science · 2020Review
- Short-Term Response of CytosolicFrontiers in plant science · 2020Article
- Role of Nitrate Reductase in NO Production in Photosynthetic Eukaryotes.Plants (Basel, Switzerland) · 2019Review
- Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis.Scientific reports · 2017Article
- Putting the N in dinoflagellates.Frontiers in microbiology · 2013Review
- Phosphorylation by MPK6: a conserved transcriptional modification mediates nitrate reductase activation and NO production?Plant signaling & behavior · 2011Article
- ATP binding to the C terminus of the Arabidopsis thaliana nitrate/proton antiporter, AtCLCa, regulates nitrate transport into plant vacuoles.The Journal of biological chemistry · 2009Article
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3 authors at 2 institutions in 1 country.
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Abstract
Several different cellular processes determine the size of the metabolically available nitrate pool in the cytoplasm. These processes include not only ion fluxes across the plasma membrane and tonoplast but also assimilation by the activity of nitrate reductase (NR). In roots, the maintenance of cytosolic nitrate activity during periods of nitrate starvation and resupply (M. van der Leij, S.J. Smith, A.J. Miller [1998] Planta 205: 64-72; R.-G. Zhen, H.-W. Koyro, R.A. Leigh, A.D. Tomos, A.J. Miller [1991] Planta 185: 356-361) suggests that this pool is regulated. Under nitrate-replete conditions vacuolar nitrate is a membrane-bound store that can release nitrate to the cytoplasm; after depletion of cytosolic nitrate, tonoplast transporters would serve to restore this pool. To study the role of assimilation, specifically the activity of NR in regulating the size of the cytosolic nitrate pool, we have compared wild-type and mutant plants. In leaf mesophyll cells, light-to-dark transitions increase cytosolic nitrate activity (1.5-2.8 mm), and these changes were reversed by dark-to-light transitions. Such changes were not observed in nia1nia2 NR-deficient plants indicating that this change in cytosolic nitrate activity was dependent on the presence of functional NR. Furthermore, in the dark, the steady-state cytosolic nitrate activities were not statistically different between the two types of plant, indicating that NR has little role in determining resting levels of nitrate. Epidermal cells of both wild type and NR mutants had cytosolic nitrate activities that were not significantly different from mesophyll cells in the dark and were unaltered by dark-to-light transitions. We propose that the NR-dependent changes in cytosolic nitrate provide a cellular mechanism for the diurnal changes in vacuolar nitrate storage, and the results are discussed in terms of the possible signaling role of cytosolic nitrate.
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