ArticleNucleic acids research2005
Internal initiation of translation of the TrkB mRNA is mediated by multiple regions within the 5' leader.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons.Scientific reports · 2017Article
- Dysregulating IRES-dependent translation contributes to overexpression of oncogenic Aurora A Kinase.Molecular cancer research : MCR · 2013Article
- Secondary RNA structure and nucleotide specificity contribute to internal initiation mediated by the human tau 5' leader.RNA biology · 2012Article
- Transcripts with in silico predicted RNA structure are enriched everywhere in the mouse brain.BMC genomics · 2012Article
- Article
- Distinct 5' UTRs regulate XIAP expression under normal growth conditions and during cellular stress.Nucleic acids research · 2010Article
- The human insulin receptor mRNA contains a functional internal ribosome entry segment.Nucleic acids research · 2009Article
- Regulating amyloid precursor protein synthesis through an internal ribosomal entry site.Nucleic acids research · 2008Article
- Identifying intrinsic and extrinsic determinants that regulate internal initiation of translation mediated by the FMR1 5' leader.BMC molecular biology · 2008Article
- Article
- Alternatively spliced isoforms of the human elk-1 mRNA within the 5' UTR: implications for ELK-1 expression.Nucleic acids research · 2007Article
- Searching for IRES.RNA (New York, N.Y.) · 2006Review
- The dicistronic RNA from the mouse LINE-1 retrotransposon contains an internal ribosome entry site upstream of each ORF: implications for retrotransposition.Nucleic acids research · 2006Article
- Dendritic BC1 RNA in translational control mechanisms.The Journal of cell biology · 2005Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Translational regulation of the dendritically localized mRNA encoding for the neurotrophin receptor TrkB has important ramifications for synaptic function. We examined whether the TrkB mRNA is translated through an internal initiation entry site (IRES). The human TrkB 5' leaders are derived from the use of alternative promoters and alternative splicing, but all 5' leaders share a common exon. Insertion of a full-length 5' leader, as well as the common exon into the intercistronic region of a dicistronic luciferase construct, yielded luciferase activity generated from the second cistron that was either equivalent or higher than that observed from the encephalomyocarditis virus IRES. Moreover, inhibiting cap-dependent translation ex vivo and in in vitro lysates had only a minimal effect on the translation of mRNA containing the TrkB 5' leader. Dissecting the 5' leader showed that the IRES is located in the exon common to all TrkB 5' leaders. Moreover, six regions ranging from 2 to 25 nt were identified that either promoted or inhibited IRES activity. Taken together, these results suggest that the 5' leader of the human TrkB mRNA contains multiple cis-elements that regulate internal initiation of translation and that this mechanism may contribute significantly to the translation of the TrkB mRNA in neuronal dendrites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.