ArticleInfection and immunity2005
Killing of dsrA mutants of Haemophilus ducreyi by normal human serum occurs via the classical complement pathway and is initiated by immunoglobulin M binding.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Dynamic immune response characteristics of piglets infected with Actinobacillus pleuropneumoniae through omic.AMB Express · 2021Article
- Interactions of the Skin PathogenFrontiers in immunology · 2020Review
- Article
- Defining Potential Vaccine Targets of Haemophilus ducreyi Trimeric Autotransporter Adhesin DsrA.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2015Article
- Rapid killing of Capnocytophaga canimorsus and Capnocytophaga cynodegmi by human whole blood and serum is mediated via the complement system.SpringerPlus · 2015Article
- Article
- Outer membrane protein P5 is required for resistance of nontypeable Haemophilus influenzae to both the classical and alternative complement pathways.Infection and immunity · 2014Article
- Trimeric autotransporter DsrA is a major mediator of fibrinogen binding in Haemophilus ducreyi.Infection and immunity · 2013Article
- Prc contributes to Escherichia coli evasion of classical complement-mediated serum killing.Infection and immunity · 2012Article
- Sialylation of lipooligosaccharides is dispensable for the virulence of Haemophilus ducreyi in humans.Infection and immunity · 2012Article
- Deletion of mtrC in Haemophilus ducreyi increases sensitivity to human antimicrobial peptides and activates the CpxRA regulon.Infection and immunity · 2011Article
- Activation of the CpxRA system by deletion of cpxA impairs the ability of Haemophilus ducreyi to infect humans.Infection and immunity · 2010Article
- Host-pathogen interplay of Haemophilus ducreyi.Current opinion in infectious diseases · 2010Review
- Localization of the domains of the Haemophilus ducreyi trimeric autotransporter DsrA involved in serum resistance and binding to the extracellular matrix proteins fibronectin and vitronectin.Infection and immunity · 2009Article
- Yersinia enterocolitica serum resistance proteins YadA and ail bind the complement regulator C4b-binding protein.PLoS pathogens · 2008Article
- Outer membrane protein DsrA is the major fibronectin-binding determinant of Haemophilus ducreyi.Infection and immunity · 2008Article
- Dysregulated immune profiles for skin and dendritic cells are associated with increased host susceptibility to Haemophilus ducreyi infection in human volunteers.Infection and immunity · 2007Article
- Immunization with the Haemophilus ducreyi hemoglobin receptor HgbA protects against infection in the swine model of chancroid.Infection and immunity · 2006Article
- A DltA mutant of Haemophilus ducreyi Is partially attenuated in its ability to cause pustules in human volunteers.Infection and immunity · 2006Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Previously, we showed that serum resistance in Haemophilus ducreyi type strain 35000HP required expression of the outer membrane protein DsrA because the isogenic dsrA mutant FX517 is highly serum susceptible. In this study, we confirmed this finding by construction of additional serum-susceptible dsrA mutants in more recently isolated serum-resistant strains. We also demonstrated that killing of dsrA mutants required an intact classical complement cascade but not the alternative or mannan-binding lectin pathways. Between 5- and 10-fold more purified human immunoglobulin M (IgM) but not IgG was deposited onto dsrA mutant FX517 than onto parent strain 35000HP, consistent with IgM initiation of the classical cascade. Depletion of IgM, but not IgG, from complement-intact serum inhibited killing of FX517. As predicted from the amounts of IgM bound, more of the individual complement components were bound by FX517 than by parent strain 35000HP. Examination of the binding of negative regulators of complement as an explanation for serum resistance indicated that parent strain 35000HP bound more C4 binding protein and vitronectin than FX517 but not factor H. However, the degree and pattern of complement component binding observed suggested that IgM binding to the serum-susceptible mutant FX517 was responsible for the activation of the classical pathway and the observed killing of FX517 as opposed to binding of negative regulators of complement by the serum-resistant parent. We speculate that an undefined neo-epitope, possibly carbohydrate, is exposed in the dsrA mutant that is recognized by naturally occurring bactericidal IgM antibodies present in human sera.
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