ArticleInfection and immunity2005
Entamoeba histolytica and Entamoeba dispar utilize externalized phosphatidylserine for recognition and phagocytosis of erythrocytes.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
- Drug repositioning: antiprotozoal activity of terfenadine against Entamoeba histolytica trophozoites.Parasitology research · 2022Article
- Apoptotic mimicry as a strategy for the establishment of parasitic infections: parasite- and host-derived phosphatidylserine as key molecule.Cell communication and signaling : CCS · 2020Review
- Influence of Micropatterned Grill Lines onFrontiers in cellular and infection microbiology · 2018Article
- The Physiology of Phagocytosis in the Context of Mitochondrial Origin.Microbiology and molecular biology reviews : MMBR · 2017Review
- Entamoeba histolytica: Host parasite interactions at the colonic epithelium.Tissue barriers · 2017Article
- Endoplasmic reticulum-resident Rab8A GTPase is involved in phagocytosis in the protozoan parasite Entamoeba histolytica.Cellular microbiology · 2016Article
- Knockdown of Five Genes Encoding Uncharacterized Proteins Inhibits Entamoeba histolytica Phagocytosis of Dead Host Cells.Infection and immunity · 2016Article
- Chew on this: amoebic trogocytosis and host cell killing by Entamoeba histolytica.Trends in parasitology · 2015Review
- Parasites and their heterophagic appetite for disease.PLoS pathogens · 2015Review
- Erythrophagocytosis in Entamoeba histolytica and Entamoeba dispar: a comparative study.BioMed research international · 2014Article
- Feed-forward regulation of phagocytosis by Entamoeba histolytica.Infection and immunity · 2012Article
- A Toll/IL-1R/resistance domain-containing thioredoxin regulates phagocytosis in Entamoeba histolytica.Parasites & vectors · 2012Article
- Entamoeba histolytica cell surface calreticulin binds human c1q and functions in amebic phagocytosis of host cells.Infection and immunity · 2012Article
- Exposure to host ligands correlates with colocalization of Gal/GalNAc lectin subunits in lipid rafts and phosphatidylinositol (4,5)-bisphosphate signaling in Entamoeba histolytica.Eukaryotic cell · 2012Article
- Leptin protects host cells from Entamoeba histolytica cytotoxicity by a STAT3-dependent mechanism.Infection and immunity · 2012Article
- Common pathways for receptor-mediated ingestion of Escherichia coli and LDL cholesterol by Entamoeba histolytica regulated in part by transmembrane kinase 39.International journal for parasitology · 2012Article
- Localisation to lipid rafts correlates with increased function of the Gal/GalNAc lectin in the human protozoan parasite, Entamoeba histolytica.International journal for parasitology · 2011Article
- Calpains are involved in Entamoeba histolytica-induced death of HT-29 colonic epithelial cells.The Korean journal of parasitology · 2011Article
- Evidence for a novel Entamoeba histolytica lectin activity that recognises carbohydrates present on ovalbumin.International journal for parasitology · 2011Article
- A Sequential Model of Host Cell Killing and Phagocytosis by Entamoeba histolytica.Journal of parasitology research · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Amebic erythrophagocytosis is characteristic of invasive amebiasis, and mutants deficient in erythrocyte ingestion are avirulent. We sought to understand the molecular mechanisms underlying erythrocyte phagocytosis by Entamoeba histolytica. Following adherence to amebae, erythrocytes became round and crenulated, and phosphatidylserine (PS) was exposed on their outer membrane leaflets. These changes were similar to the effects of calcium treatment on erythrocytes, which we utilized to separate ameba-induced exposure of erythrocyte PS from the process of phagocytosis. The adherence and phagocytosis of calcium-treated erythrocytes were less inhibited by galactose than were those of healthy erythrocytes, suggesting the existence of an amebic coreceptor specific for PS. To test whether PS was recognized by amebae, calcium-treated cells were incubated with annexin V prior to adherence to or ingestion by E. histolytica. Annexin V blocked both adherence (50% +/- 12% inhibition; P < 0.05) and phagocytosis (65% +/- 10%; P < 0.05), providing evidence that at least one galactose-independent coreceptor was involved in the adherence and ingestion of red blood cells. The coreceptor was inhibited by phospho-l-serine and to a lesser extent by phospho-d-serine but not by phospho-l-threonine, which is consistent with the coreceptor functioning in the adherence and ingestion of erythrocytes via recognition of PS. We expanded our investigations to the highly related but noninvasive parasite Entamoeba dispar and demonstrated that it was deficient in red-blood-cell adherence, induction of PS exposure, and phagocytosis. These findings establish phosphatidylserine involvement in erythrophagocytosis by amebae and suggest the existence of a PS receptor on the surfaces of both E. histolytica and E. dispar.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.