ArticlePharmaceutical research2005
Intracellular delivery of Bak BH3 peptide by microbubble-enhanced ultrasound.
Article in Pharmaceutical research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Using focused ultrasound to modulate microglial structure and function.Frontiers in cellular neuroscience · 2023Review
- Making waves: how ultrasound-targeted drug delivery is changing pharmaceutical approaches.Materials advances · 2022Review
- Platelet Membrane Biomimetic Nanoparticles Combined With UTMD to Improve the Stability of Atherosclerotic Plaques.Frontiers in chemistry · 2022Article
- Mitigation of Articular Cartilage Degeneration and Subchondral Bone Sclerosis in Osteoarthritis Progression Using Low-Intensity Ultrasound Stimulation.Ultrasound in medicine & biology · 2019Article
- Concurrent Treatment with Anti-DLL4 Enhances Antitumor and Proapoptotic Efficacy of a γ-Secretase Inhibitor in Gastric Cancer.Translational oncology · 2018Article
- Selective recapitulation of conserved and nonconserved regions of putative NOXA1 protein activation domain confers isoform-specific inhibition of Nox1 oxidase and attenuation of endothelial cell migration.The Journal of biological chemistry · 2013Article
- Ultrasound-based molecular imaging and specific gene delivery to mesenteric vasculature by endothelial adhesion molecule targeted microbubbles in a mouse model of Crohn's disease.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Can ultrasound enable efficient intracellular uptake of molecules? A retrospective literature review and analysis.Ultrasound in medicine & biology · 2012Review
- Article
- Production of uniformly sized serum albumin and dextrose microbubbles.Ultrasonics sonochemistry · 2012Article
- Expression of endostatin mediated by a novel non-viral delivery system inhibits human umbilical vein endothelial cells in vitro.Molecular biology reports · 2010Article
- Gold nanoparticles delivery in mammalian live cells: a critical review.Nano reviews · 2010Article
- Ultrasound triggered cell death in vitro with doxorubicin loaded poly lactic-acid contrast agents.Ultrasonics · 2009Article
- Key factors that affect sonoporation efficiency in in vitro settings: the importance of standing wave in sonoporation.Biochemical and biophysical research communications · 2007Article
- Activation of Bak in ultrasound-induced, JNK- and p38-independent apoptosis and its inhibition by Bcl-2.Biochemical and biophysical research communications · 2007Article
- Long-circulating liposomes radiolabeled with [18F]fluorodipalmitin ([18F]FDP).Nuclear medicine and biology · 2007Article
- Ultrasound Microbubble Delivery Targeting Intraplaque Neovascularization Inhibits Atherosclerotic Plaque in an APOE-deficient Mouse Model.In vivo (Athens, Greece)Article
- Ultrasound imaging beyond the vasculature with new generation contrast agents.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo investigate the possibility of intracellular delivery of Bak BH3 peptide using sonoporation effect by microbubble-enhanced ultrasound.
methodsHeLa and BJAB cells were exposed to 1.696-Mhz focused ultrasound with 2% microbubble contrast agents (OPTISON). Cell-impermeable calcein was used as an indicator for successful sonoporation, and propidium iodide staining was used for cell viability assessment. Peptides were also exposed to ultrasound with OPTISON and analyzed with mass spectrometry for evaluation of stability under ultrasound exposure. The effect of transduced Bak BH3 peptide was evaluated by the cell viability of successfully sonoporated cells.
resultsBak BH3 peptides did not undergo mechanical degradation with microbubble-enhanced ultrasound exposure. With the increase of acoustic energy exposure, the sonoporation efficiency saturated both in BJAB and HeLa cells, while direct cell death rate by ultrasound exposure tended to increase. When BJAB cells were treated with 100 microM Bak BH3 peptides, and ultrasound exposure with ultrasound contrast agents (OPTISON), an increased 35% cell death was confirmed. On the other hand, although HeLa cells had a similar trend, they failed to exhibit statistical significance.
conclusionsOur results suggest that microbubble-enhanced focused ultrasound peptide transduction is possible. Further optimization of ultrasound exposure conditions may be necessary.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.