ArticleJournal of bioenergetics and biomembranes2005
A possible site of superoxide generation in the complex I segment of rat heart mitochondria.
Article in Journal of bioenergetics and biomembranes, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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Who cites it
43 citing papers in PubMed, 122 citations in OpenAlex.
- Mitochondrial redox regulation and myocardial ischemia-reperfusion injury.American journal of physiology. Cell physiology · 2022Review
- Molecular and Supramolecular Structure of the Mitochondrial Oxidative Phosphorylation System: Implications for Pathology.Life (Basel, Switzerland) · 2021Review
- Generation of Reactive Oxygen Species by Mitochondria.Antioxidants (Basel, Switzerland) · 2021Review
- Inhibition of the ubiquitous calpains protects complex I activity and enables improved mitophagy in the heart following ischemia-reperfusion.American journal of physiology. Cell physiology · 2019Article
- Membrane potential and delta pH dependency of reverse electron transport-associated hydrogen peroxide production in brain and heart mitochondria.Journal of bioenergetics and biomembranes · 2018Article
- Reverse electron transfer results in a loss of flavin from mitochondrial complex I: Potential mechanism for brain ischemia reperfusion injury.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2017Article
- Melatonin and the electron transport chain.Cellular and molecular life sciences : CMLS · 2017Review
- Mitochondrial Dysfunction and Myocardial Ischemia-Reperfusion: Implications for Novel Therapies.Annual review of pharmacology and toxicology · 2017Review
- The unique chemistry and biology of the piericidins.The Journal of antibiotics · 2016Review
- Ischemic A/D transition of mitochondrial complex I and its role in ROS generation.Biochimica et biophysica acta · 2016Review
- Reactive oxygen species production in cardiac mitochondria after complex I inhibition: Modulation by substrate-dependent regulation of the NADH/NAD(+) ratio.Free radical biology & medicine · 2016Article
- Mitochondrial Metabolism in Aging Heart.Circulation research · 2016Review
- The Role of Mitochondrial Reactive Oxygen Species in Cardiovascular Injury and Protective Strategies.Oxidative medicine and cellular longevity · 2016Review
- Idebenone and neuroprotection: antioxidant, pro-oxidant, or electron carrier?Journal of bioenergetics and biomembranes · 2015Review
- Transient complex I inhibition at the onset of reperfusion by extracellular acidification decreases cardiac injury.American journal of physiology. Cell physiology · 2014Article
- Cardiac mitochondria and reactive oxygen species generation.Circulation research · 2014Review
- Fatty acids in energy metabolism of the central nervous system.BioMed research international · 2014Review
- Reverse electron flow-mediated ROS generation in ischemia-damaged mitochondria: role of complex I inhibition vs. depolarization of inner mitochondrial membrane.Biochimica et biophysica acta · 2013Article
- Complex I and ATP synthase mediate membrane depolarization and matrix acidification by isoflurane in mitochondria.European journal of pharmacology · 2012Article
- Age-related decline in mitochondrial bioenergetics: does supercomplex destabilization determine lower oxidative capacity and higher superoxide production?Seminars in cell & developmental biology · 2012Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
We searched for possible sites of superoxide generation in the complex I segment of the respiratory chain by studying both forward and reverse electron transfer reactions in isolated rat heart mitochondria. Superoxide production was monitored by measuring the release of hydrogen peroxide from mitochondria with a fluorescence spectrophotometer using the Amplex red/horseradish peroxidase system. In the forward electron transfer, a slow superoxide production in the presence of glutamate and malate was enhanced by both rotenone and piericidin A (specific inhibitors at the end of the complex I respiratory chain). Both diphenileneiodonium and ethoxyformic anhydride (inhibitors for respiratory components located upstream of the respiratory chain) inhibited the enhancement by rotenone and piericidin A. In contrast, in reverse electron transfer driven by ATP, both diphenileneiodonium and ethoxyformic anhydride enhanced the superoxide production. Piericidin A also increased superoxide production. Rotenone increased it only in the presence of piericidin A. Our results suggest that the major site of superoxide generation is not flavin, but protein-associated ubisemiquinones which are spin-coupled with iron-sulfur cluster N2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.