ArticleGenes & development2005
A human splicing factor, SKIP, associates with P-TEFb and enhances transcription elongation by HIV-1 Tat.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 115 citations in OpenAlex.
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- Histone H2B monoubiquitylation regulates elongation-to-termination transition in RNA polymerase II transcription.Communications biology · 2025Article
- Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes.RNA biology · 2024Article
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- A pilot study to show that asymptomatic sexually transmitted infections alter the foreskin epithelial proteome.Frontiers in microbiology · 2022Article
- Conditional depletion of transcriptional kinases Ctk1 and Bur1 and effects on co-transcriptional spliceosome assembly and pre-mRNA splicing.RNA biology · 2021Article
- Splicing factor USP39 promotes ovarian cancer malignancy through maintaining efficient splicing of oncogenic HMGA2.Cell death & disease · 2021Article
- Gene Architecture Facilitates Intron-Mediated Enhancement of Transcription.Frontiers in molecular biosciences · 2021Review
- Skip is essential for Notch signaling to induce Sox2 in cerebral arteriovenous malformations.Cellular signalling · 2020Article
- Spt5 modulates cotranscriptional spliceosome assembly inRNA (New York, N.Y.) · 2019Article
- Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana.Nucleic acids research · 2019Article
- SNW1, a Novel Transcriptional Regulator of the NF-κB Pathway.Molecular and cellular biology · 2019Article
- To Splice or to Transcribe: SKIP-Mediated Environmental Fitness and Development in Plants.Frontiers in plant science · 2019Review
- The HIV-1 Tat Protein Enhances Splicing at the Major Splice Donor Site.Journal of virology · 2018Article
- Nineteen complex-related factor Prp45 is required for the early stages of cotranscriptional spliceosome assembly.RNA (New York, N.Y.) · 2017Article
- Role of Host Factors on the Regulation of Tat-Mediated HIV-1 Transcription.Current pharmaceutical design · 2017Review
- A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans.PLoS genetics · 2016Article
- Coupling of RNA Polymerase II Transcription Elongation with Pre-mRNA Splicing.Journal of molecular biology · 2016Review
- p53-derived host restriction of HIV-1 replication by protein kinase R-mediated Tat phosphorylation and inactivation.Journal of virology · 2015Article
- Inhibition of SNW1 association with spliceosomal proteins promotes apoptosis in breast cancer cells.Cancer medicine · 2015Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HIV-1 Tat binds human CyclinT1 and recruits the CDK9/P-TEFb complex to the viral TAR RNA in a step that links RNA polymerase II (RNAPII) C-terminal domain (CTD) Ser 2 phosphorylation with transcription elongation. Previous studies have suggested a connection between Tat and pre-mRNA splicing factors. Here we show that the splicing-associated c-Ski-interacting protein, SKIP, is required for Tat transactivation in vivo and stimulates HIV-1 transcription elongation, but not initiation, in vitro. SKIP associates with CycT1:CDK9/P-TEFb and Tat:P-TEFb complexes in nuclear extracts and interacts with recombinant Tat:P-TEFb:TAR RNA complexes in vitro, indicating that it may act through nascent RNA to overcome pausing by RNAPII. SKIP also associates with U5snRNP proteins and tri-snRNP110K in nuclear extracts, and facilitates recognition of an alternative Tat-specific splice site in vivo. The effects of SKIP on transcription elongation, binding to P-TEFb, and splicing are mediated through the SNW domain. HIV-1 Tat transactivation is accompanied by the recruitment of P-TEFb, SKIP, and tri-snRNP110K to the integrated HIV-1 promoter in vivo, whereas the U5snRNPs associate only with the transcribed coding region. These findings suggest that SKIP plays independent roles in transcription elongation and pre-mRNA splicing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.