ArticleThe Journal of physiology2005
Different effects of cardiac versus skeletal muscle regulatory proteins on in vitro measures of actin filament speed and force.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Gene Therapy Rescues Cardiac Dysfunction in Duchenne Muscular Dystrophy Mice by Elevating Cardiomyocyte Deoxy-Adenosine Triphosphate.JACC. Basic to translational science · 2019Article
- Physiological Mitochondrial Fragmentation Is a Normal Cardiac Adaptation to Increased Energy Demand.Circulation research · 2018Article
- Molecular mechanisms underlying deoxy-ADP.Pi activation of pre-powerstroke myosin.Protein science : a publication of the Protein Society · 2017Article
- Role of cardiac troponin I carboxy terminal mobile domain and linker sequence in regulating cardiac contraction.Archives of biochemistry and biophysics · 2016Article
- Contractile properties of developing human fetal cardiac muscle.The Journal of physiology · 2016Article
- Impact of tropomyosin isoform composition on fast skeletal muscle thin filament regulation and force development.Journal of muscle research and cell motility · 2015Article
- Contractility and kinetics of human fetal and human adult skeletal muscle.The Journal of physiology · 2013Article
- The effects of slow skeletal troponin I expression in the murine myocardium are influenced by development-related shifts in myosin heavy chain isoform.The Journal of physiology · 2012Article
- Correlation of molecular and functional effects of mutations in cardiac troponin T linked to familial hypertrophic cardiomyopathy: an integrative in silico/in vitro approach.The Journal of biological chemistry · 2012Article
- Tropomyosin flexural rigidity and single ca(2+) regulatory unit dynamics: implications for cooperative regulation of cardiac muscle contraction and cardiomyocyte hypertrophy.Frontiers in physiology · 2012Article
- The role of tropomyosin domains in cooperative activation of the actin-myosin interaction.Journal of molecular biology · 2011Article
- Myofilament length dependent activation.Journal of molecular and cellular cardiology · 2010Review
- Investigations into tropomyosin function using mouse models.Journal of molecular and cellular cardiology · 2010Review
- The cardiac troponin C mutation Leu29Gln found in a patient with hypertrophic cardiomyopathy does not alter contractile parameters in skinned murine myocardium.Basic research in cardiology · 2009Article
- Differences between cardiac and skeletal troponin interaction with the thin filament probed by troponin exchange in skeletal myofibrils.Biophysical journal · 2009Article
- Rescue of skeletal muscle alpha-actin-null mice by cardiac (fetal) alpha-actin.The Journal of cell biology · 2009Article
- Cooperative cross-bridge activation of thin filaments contributes to the Frank-Starling mechanism in cardiac muscle.Biophysical journal · 2009Article
- Phosphorylation of tropomyosin extends cooperative binding of myosin beyond a single regulatory unit.Cell motility and the cytoskeleton · 2009Article
- Thin filament Ca2+ binding properties and regulatory unit interactions alter kinetics of tension development and relaxation in rabbit skeletal muscle.The Journal of physiology · 2008Article
- Sarcomere lattice geometry influences cooperative myosin binding in muscle.PLoS computational biology · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mammalian cardiac and skeletal muscle express unique isoforms of the thin filament regulatory proteins, troponin (Tn) and tropomyosin (Tm), and the significance of these different isoforms in thin filament regulation has not been clearly identified. Both in vitro and skinned cellular studies investigating the mechanism of thin filament regulation in striated muscle have often used heterogeneous mixtures of Tn, Tm and myosin isoforms, and variability in reported results might be explained by different combinations of these proteins. Here we used in vitro motility and force (microneedle) assays to investigate the influence of cardiac versus skeletal Tn and Tm isoforms on actin-heavy meromyosin (HMM) mechanics. When interacting with skeletal HMM, thin filaments reconstituted with cardiac Tn/Tm or skeletal Tn/Tm exhibited similar speed-calcium relationships and significantly increased maximum speed and force per filament length (F/l) at pCa 5 (versus unregulated actin filaments). However, augmentation of F/l was greater with skeletal regulatory proteins. Reconstitution of thin filaments with the heterogeneous combination of skeletal Tn and cardiac Tm decreased sliding speeds at all [Ca2+] relative to thin filaments with skeletal Tn/Tm. Finally, for filaments reconstituted with any heterogeneous mix of Tn and Tm isoforms, force was not potentiated over that of unregulated actin filaments. Combined the results suggest (1) that cardiac regulatory proteins limit the allosteric enhancement of force, and (2) that Tn and Tm isoform homogeneity is important when studying Ca2+ regulation of crossbridge binding and kinetics as well as mechanistic differences between cardiac and skeletal muscle.
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