ArticleWorld journal of gastroenterology2005
Multigene tracking of quasispecies in viral persistence and clearance of hepatitis C virus.
Article in World journal of gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Deglycosylation of hepatitis C virus pseudoparticles with 2-3,6,8,9 Neuraminidase A elicits protective neutralizing antibodies in mice.Scientific reports · 2026Article
- A Novel Information-Theory-Based Genetic Distance That Approximates Phenotypic Differences.Journal of computational biology : a journal of computational molecular cell biology · 2023Article
- Evidence of distinct populations of hepatitis C virus in the liver and plasma of patients co-infected with HIV and HCV.Journal of medical virology · 2014Article
- Acute hepatitis C: management in the rapidly evolving world of HCV.Current gastroenterology reports · 2014Review
- Persistence of rhinovirus RNA and IP-10 gene expression after acute asthma.Respirology (Carlton, Vic.) · 2011Article
- Full-length characterization of hepatitis C virus subtype 3a reveals novel hypervariable regions under positive selection during acute infection.Journal of virology · 2009Article
- Directly transmitted viral diseases: modeling the dynamics of transmission.Trends in microbiology · 2008Review
- Influence of quasispecies on virological responses and disease severity in patients with chronic hepatitis C.World journal of gastroenterology · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the evaluation of hepatitis C virus (HCV) quasispecies in the envelope region and its relationship with the outcome of acute hepatitis C.
methodsHCV quasispecies were characterized in specimens collected every 2-6 mo from a cohort of acutely HCV-infected subjects. We evaluated two individuals who spontaneously cleared viremia and three individuals with persistent viremia by cloning 33 1-kb amplicons that spanned E1 and the 5' half of E2, including hypervariable region 1 (HVR1). To assess the quasispecies complexity and to detect variants for sequencing, 33 cloned cDNAs representing each specimen were assessed by a combined method of analysis of a single-stranded conformational polymorphism and heteroduplex analysis. The rates of both synonymous and nonsynonymous substitutions for the E1, HVR1 and E2 regions outside HVR1 were analyzed.
resultsSerum samples collected from chronic phase of infection had higher quasispecies complexity than those collected from acute phase of infection in all individuals examined. The genetic diversity (genetic distance) within HVR1 was consistently higher than that in the complete E1 (0.0322+/-0.0068 vs -0.0020+/-0.0014, P<0.05) and E2 regions outside HVR1 (0.0322+/-0.0068 vs 0.0017+/-0.0011, P<0.05) in individuals with persistent viremia, but did not change markedly over time in those with clearance of viremia. For individuals with persistent viremia, the rate of nonsynonymous substitutions within the HVR1 region (2.76X10(-3)+/-1.51X10(-3)) predominated and gradually increased, as compared with that in the E1 and E2 regions outside HVR1 (0.23X10(-3)+/-0.15X10(-3), 0.50X10(-3)+/-0.10X10(-3)). By contrast, the rates of both nonsynonymous and synonymous substitutions for the E1 and E2 regions including HVR1 were consistently lower in individuals with clearance of viremia.
conclusionHCV persistence is associated with a complexity quasispecies and positive selection of HVR1 by the host immune system.
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