ArticleThe EMBO journal2005
The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins.
Article in The EMBO journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 79 citations in OpenAlex.
- Spatio-temporal organization of theEcoSal Plus · 2024Review
- Natural flavonoid pectolinarin computationally targeted as a promising drug candidate against SARS-CoV-2.Current research in structural biology · 2024Article
- The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.Molecular cell · 2022Article
- Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites.Molecular cell · 2021Article
- Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.Nature communications · 2021Article
- Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.Molecular cell · 2020Article
- Chromosome organization in bacteria: mechanistic insights into genome structure and function.Nature reviews. Genetics · 2020Review
- Architecture of the Escherichia coli nucleoid.PLoS genetics · 2019Article
- Dynamic architecture of the Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBEF.Nucleic acids research · 2019Article
- Article
- Towards a Unified Model of SMC Complex Function.Current biology : CB · 2018Review
- A new twist in the coil: functions of the coiled-coil domain of structural maintenance of chromosome (SMC) proteins.Current genetics · 2018Review
- MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin.eLife · 2018Article
- Condensin-mediated chromosome organization in fission yeast.Current genetics · 2016Review
- In vitro topological loading of bacterial condensin MukB on DNA, preferentially single-stranded DNA rather than double-stranded DNA.Scientific reports · 2016Article
- Interaction between TBP and Condensin Drives the Organization and Faithful Segregation of Mitotic Chromosomes.Molecular cell · 2015Article
- Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis.Chromosoma · 2015Article
- A novel role for the condensin II complex in cellular senescence.Cell cycle (Georgetown, Tex.) · 2015Article
- MukBEF, a chromosomal organizer.Journal of molecular microbiology and biotechnology · 2014Review
- Breaking symmetry in SMCs.Nature structural & molecular biology · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Escherichia coli MukB, MukE, and MukF proteins form a bacterial condensin (MukBEF) that contributes to chromosome management by compacting DNA. MukB is an ATPase and DNA-binding protein of the SMC superfamily; however, the structure and function of non-SMC components, such as MukF, have been less forthcoming. Here, we report the crystal structure of the N-terminal 287 amino acids of MukF at 2.9 A resolution. This region folds into a winged-helix domain and an extended coiled-coil domain that self-associate to form a stable, doubly domain-swapped dimer. Protein dissection and affinity purification data demonstrate that the region of MukF C-terminal to this fragment binds to MukE and MukB. Our findings, together with sequence analyses, indicate that MukF is a kleisin subunit for E. coli condensin and suggest a means by which it may organize the MukBEF assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.