ArticleJournal of bacteriology2005
A multidomain fusion protein in Listeria monocytogenes catalyzes the two primary activities for glutathione biosynthesis.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 102 citations in OpenAlex.
- Heterogeneity in the response to the metabolic environment drives fosfomycin heteroresistance.Nature communications · 2026Article
- Effects of Glutathione on Antibiotic Susceptibility and Resistance in Bacteria: A Comprehensive Review.Infection and drug resistance · 2026Review
- Stress-dependent activation of themBio · 2025Article
- C-di-AMP accumulation disrupts glutathione metabolism inInfection and immunity · 2024Article
- Identification of a glutathione transporter inMicrobiology spectrum · 2024Article
- Article
- How Bacterial Pathogens Coordinate Appetite with Virulence.Microbiology and molecular biology reviews : MMBR · 2023Review
- The Glutathione System: A Journey from Cyanobacteria to Higher Eukaryotes.Antioxidants (Basel, Switzerland) · 2023Review
- Characterization of GshAB of Tetragenococcus halophilus: a two-domain glutathione synthetase.Applied microbiology and biotechnology · 2023Article
- Review
- Article
- Article
- Inhibition of the master regulator of Listeria monocytogenes virulence enables bacterial clearance from spacious replication vacuoles in infected macrophages.PLoS pathogens · 2022Article
- New roles for glutathione: Modulators of bacterial virulence and pathogenesis.Redox biology · 2021Article
- The DUG Pathway Governs Degradation of Intracellular Glutathione in Aspergillus nidulans.Applied and environmental microbiology · 2021Article
- Alternative σ Factors Regulate Overlapping as Well as Distinct Stress Response and Metabolic Functions inPathogens (Basel, Switzerland) · 2021Article
- The protective role of intracellular glutathione in Saccharomyces cerevisiae during lignocellulosic ethanol production.AMB Express · 2020Article
- Bacterial Metabolites of Human Gut Microbiota Correlating with Depression.International journal of molecular sciences · 2020Review
- Article
- Redox and Thiols in Archaea.Antioxidants (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutathione is the predominant low-molecular-weight peptide thiol present in living organisms and plays a key role in protecting cells against oxygen toxicity. Until now, glutathione synthesis was thought to occur solely through the consecutive action of two physically separate enzymes, gamma-glutamylcysteine ligase and glutathione synthetase. In this report we demonstrate that Listeria monocytogenes contains a novel multidomain protein (termed GshF) that carries out complete synthesis of glutathione. Evidence for this comes from experiments which showed that in vitro recombinant GshF directs the formation of glutathione from its constituent amino acids and the in vivo effect of a mutation in GshF that abolishes glutathione synthesis, results in accumulation of the intermediate gamma-glutamylcysteine, and causes hypersensitivity to oxidative agents. We identified GshF orthologs, consisting of a gamma-glutamylcysteine ligase (GshA) domain fused to an ATP-grasp domain, in 20 gram-positive and gram-negative bacteria. Remarkably, 95% of these bacteria are mammalian pathogens. A plausible origin for GshF-dependent glutathione biosynthesis in these bacteria was the recruitment by a GshA ancestor gene of an ATP-grasp gene and the subsequent spread of the fusion gene between mammalian hosts, most likely by horizontal gene transfer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.