ArticleJournal of bacteriology2005
Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 144 citations in OpenAlex.
- Bacteria producing the bioplastic polyhydroxybutyrate kill the nematode Caenorhabditis elegans.PLoS biology · 2026Article
- Photovoltaic Microorganism Hybrid Systems for Enhanced Polyhydroxybutyrate Synthesis Through Material Design and Energy Mass Transfer Mechanisms.Materials (Basel, Switzerland) · 2025Review
- Rieske Oxygenase-Catalyzed Biotransformations in Recombinant Cupriavidus necator Fueled by Formate Oxidation.Chembiochem : a European journal of chemical biology · 2025Article
- Estimation of PHA concentrations from cell density data in Cupriavidus necator.Applied microbiology and biotechnology · 2025Article
- Production of Poly(3-hydroxybutyrate-Bioengineering (Basel, Switzerland) · 2024Article
- Carbon dioxide valorization into resveratrol via lithoautotrophic fermentation using engineered Cupriavidus necator H16.Microbial cell factories · 2024Article
- Extracellular Poly(hydroxybutyrate) Bioplastic Production Using Surface Display Techniques.ACS materials Au · 2024Article
- Biosynthesis of polyhydroxybutyrate byFrontiers in plant science · 2024Article
- Minimizing the Lag Phase of Cupriavidus necator Growth under Autotrophic, Heterotrophic, and Mixotrophic Conditions.Applied and environmental microbiology · 2023Article
- Co-Culture of Halotolerant Bacteria to Produce Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Using Sewage Wastewater Substrate.Polymers · 2022Article
- In vivo quantification of polyhydroxybutyrate (PHB) in the alphaproteobacterial methanotroph, Methylocystis sp. Rockwell.Applied microbiology and biotechnology · 2022Article
- The Synthesis, Characterization and Applications of Polyhydroxyalkanoates (PHAs) and PHA-Based Nanoparticles.Polymers · 2021Review
- Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediol.Metabolic engineering · 2021Article
- Imaging and modelling of poly(3-hydroxybutyrate) synthesis in Paracoccus denitrificans.AMB Express · 2021Article
- Article
- Three-dimensional label-free visualization and quantification of polyhydroxyalkanoates in individual bacterial cell in its native state.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Analysis of Polyhydroxyalkanoates Granules inPolymers · 2021Article
- Wide range of metabolic adaptations to the acquisition of the Calvin cycle revealed by comparison of microbial genomes.PLoS computational biology · 2021Article
- Polysaccharide length affects mycobacterial cell shape and antibiotic susceptibility.Science advances · 2020Article
- New insight into poly (3-hydroxybutyrate) production by Azomonas macrocytogenes isolate KC685000: large scale production, kinetic modeling, recovery and characterization.Molecular biology reports · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Wautersia eutropha, formerly known as Ralstonia eutropha, a gram-negative bacterium, accumulates polyhydroxybutyrate (PHB) as insoluble granules inside the cell when nutrients other than carbon are limited. In this paper, we report findings from kinetic studies of granule formation and degradation in W. eutropha H16 obtained using transmission electron microscopy (TEM). In nitrogen-limited growth medium, the phenotype of the cells at the early stages of granule formation was revealed for the first time. At the center of the cells, dark-stained "mediation elements" with small granules attached were observed. These mediation elements are proposed to serve as nucleation sites for granule initiation. TEM images also revealed that when W. eutropha cells were introduced into nitrogen-limited medium from nutrient-rich medium, the cell size increased two- to threefold, and the cells underwent additional volume changes during growth. Unbiased stereology was used to analyze the two-dimensional TEM images, from which the average volume of a W. eutropha H16 cell and the total surface area of granules per cell in nutrient-rich and PHB production media were obtained. These parameters were essential in the calculation of the concentration of proteins involved in PHB formation and utilization and their changes with time. The extent of protein coverage of the granule surface area is presented in the accompanying paper.
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