ArticleJournal of bacteriology2005
Characterization of methionine export in Corynebacterium glutamicum.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 89 citations in OpenAlex.
- Metabolic engineering strategies of Corynebacterium glutamicum for sulfur-containing amino acid production: current progress and future directions.World journal of microbiology & biotechnology · 2026Review
- Enhanced l-serine synthesis inSynthetic and systems biotechnology · 2025Article
- Impact of exporter proteins and their engineering on the productivity of Corynebacterium.Applied microbiology and biotechnology · 2025Review
- Engineering of Shikimate Pathway and Terminal Branch for Efficient Production of L-Tryptophan inInternational journal of molecular sciences · 2023Article
- An expanded whole-cell model of E. coli links cellular physiology with mechanisms of growth rate control.NPJ systems biology and applications · 2022Article
- CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an L-proline exporter for L-proline hyperproduction.Nature communications · 2022Article
- Topological analyses of the L-lysine exporter LysO reveal a critical role for a conserved pair of intramembrane solvent-exposed acidic residues.The Journal of biological chemistry · 2021Article
- Microbial methionine transporters and biotechnological applications.Applied microbiology and biotechnology · 2021Review
- Obtaining a series of native gradient promoter-5'-UTR sequences in Corynebacterium glutamicum ATCC 13032.Microbial cell factories · 2020Article
- High-yield production of L-serine through a novel identified exporter combined with synthetic pathway in Corynebacterium glutamicum.Microbial cell factories · 2020Article
- Contribution of mechanosensitive channels to osmoadaptation and ectoine excretion in Halomonas elongata.Extremophiles : life under extreme conditions · 2020Article
- One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst.Scientific reports · 2018Article
- A Novel Corynebacterium glutamicum l-Glutamate Exporter.Applied and environmental microbiology · 2018Article
- Metabolite secretion in microorganisms: the theory of metabolic overflow put to the test.Metabolomics : Official journal of the Metabolomic Society · 2018Review
- Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics.Scientific reports · 2018Article
- Updates on industrial production of amino acids using Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2016Review
- Controlling the transcription levels of argGH redistributed L-arginine metabolic flux in N-acetylglutamate kinase and ArgR-deregulated Corynebacterium crenatum.Journal of industrial microbiology & biotechnology · 2016Article
- YjeH Is a Novel Exporter of l-Methionine and Branched-Chain Amino Acids in Escherichia coli.Applied and environmental microbiology · 2015Article
- Anaerobic growth of Corynebacterium glutamicum via mixed-acid fermentation.Applied and environmental microbiology · 2015Article
- Efflux systems in bacteria and their metabolic engineering applications.Applied microbiology and biotechnology · 2015Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Corynebacterium glutamicum is known for its effective excretion of amino acids under particular metabolic conditions. Concomitant activities of uptake and excretion systems would create an energy-wasting futile cycle; amino acid export systems are therefore tightly regulated. We have used a DNA microarray approach to identify genes for membrane proteins which are overexpressed under conditions of elevated cytoplasmic concentrations of methionine. One of these genes was brnF, coding for the larger subunit of BrnFE, a previously identified two-component isoleucine export system. By deletion, complementation, and overexpression of the brnFE genes in a C. glutamicum strain, in which the two uptake systems for methionine were inactivated, we identified BrnFE as being responsible for methionine export. In the presence of both substrates in the cytoplasm, BrnFE was found to transport isoleucine and methionine at similar rates. The expression of the brnFE gene cluster depends on an Lrp-type transcription factor and was shown to be strongly induced by increasing cytoplasmic methionine concentration. Methionine was a better inducer than isoleucine, indicating that methionine rather than isoleucine might be the native substrate of BrnFE. When the synthesis of BrnFE was blocked by chloramphenicol, fast methionine export was still observed, but only at greatly increased cytoplasmic levels of this amino acid. This indicates the presence of at least one other methionine export system, presumably with low affinity but high capacity. Under conditions where cytoplasmic methionine does not exceed a concentration of 50 mM, BrnFE is the dominant export system for this amino acid.
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