ArticleJournal of bacteriology2005
Expression and site-directed mutagenesis of the lactococcal abortive phage infection protein AbiK.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 65 citations in OpenAlex.
- Neutralizing activity analysis of mimotopes against porcine epidemic diarrhea virus (PEDV) spike protein.Analytical and bioanalytical chemistry · 2026Article
- Revisiting lactococcal abortive infection-like antiphage systems: diversity, structures, and mechanisms.FEMS microbiology reviews · 2026Review
- Phage-triggered reverse transcription assembles a toxic repetitive gene from a noncoding RNA.Science (New York, N.Y.) · 2024Article
- Article
- A Novel Thermostable and Processive Reverse Transcriptase from a Group II Intron ofBiomolecules · 2023Article
- UG/Abi: a highly diverse family of prokaryotic reverse transcriptases associated with defense functions.Nucleic acids research · 2022Article
- Identification and classification of reverse transcriptases in bacterial genomes and metagenomes.Nucleic acids research · 2022Article
- Prokaryotic reverse transcriptases: from retroelements to specialized defense systems.FEMS microbiology reviews · 2021Review
- Expanding Diversity ofFrontiers in microbiology · 2021Article
- Systematic prediction of genes functionally associated with bacterial retrons and classification of the encoded tripartite systems.Nucleic acids research · 2020Article
- Oral Immunotherapy Using Probiotic Ice Cream Containing Recombinant Food-GradeJournal of immunology research · 2020Article
- The Clostridium difficile cell wall protein CwpV confers phase-variable phage resistance.Molecular microbiology · 2015Article
- Mutational Analysis of the Antitoxin in the Lactococcal Type III Toxin-Antitoxin System AbiQ.Applied and environmental microbiology · 2015Article
- Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution.Microbiology spectrum · 2015Review
- Article
- Effect of the abortive infection mechanism and type III toxin/antitoxin system AbiQ on the lytic cycle of Lactococcus lactis phages.Journal of bacteriology · 2013Article
- Lactococcal 936-type phages and dairy fermentation problems: from detection to evolution and prevention.Frontiers in microbiology · 2012Article
- A reverse transcriptase-related protein mediates phage resistance and polymerizes untemplated DNA in vitro.Nucleic acids research · 2011Article
- Lactococcal abortive infection protein AbiV interacts directly with the phage protein SaV and prevents translation of phage proteins.Applied and environmental microbiology · 2010Article
- Genomic analysis and relatedness of P2-like phages of the Burkholderia cepacia complex.BMC genomics · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Abortive infection mechanisms of Lactococcus lactis form a heterogeneous group of phage resistance systems that act after early phage gene expression. One of these systems, AbiK, aborts infection of the three most prevalent lactococcal phage groups of the dairy industry. In this study, it is demonstrated that the antiphage activity depends on the level of expression of the abiK gene and on the presence of a reverse transcriptase (RT) motif in AbiK. The abiK gene was shown to be part of an operon that includes two additional open reading frames, with one of these encoding a phage-related transcriptional repressor named Orf4. Expression of AbiK is driven by two promoters, PabiK and Porf3, the latter being repressed by Orf4 in vivo. Binding of the purified Orf4 to the Porf3 promoter was demonstrated in vitro by gel retardation assays. The N-terminal half of the deduced AbiK protein possesses an RT motif that was modified by site-directed mutagenesis. Conservative mutations in key positions resulted in the complete loss of the resistance phenotype. These data suggest that an RT activity might be involved in the phage resistance activity of AbiK. A model for the mode of action of AbiK is proposed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.