ArticleJournal of bacteriology2005
Levels and activity of the Pseudomonas putida global regulatory protein Crc vary according to growth conditions.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 58 citations in OpenAlex.
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- Degradation of Dioxins and DBF in Urban Soil Microcosms from Lausanne (Switzerland): Functional Performance of Indigenous Bacterial Strains.Microorganisms · 2025Article
- Post-Transcriptional Control in the Regulation of Polyhydroxyalkanoates Synthesis.Life (Basel, Switzerland) · 2021Review
- Rewiring the functional complexity between Crc, Hfq and sRNAs to regulate carbon catabolite repression in Pseudomonas.World journal of microbiology & biotechnology · 2019Review
- Multi-omics analysis unravels a segregated metabolic flux network that tunes co-utilization of sugar and aromatic carbons inThe Journal of biological chemistry · 2019Article
- High-Yield Production of 4-Hydroxybenzoate From Glucose or Glycerol by an EngineeredFrontiers in bioengineering and biotechnology · 2019Article
- Transcription factor levels enable metabolic diversification of single cells of environmental bacteria.The ISME journal · 2016Article
- Computational prediction of the Crc regulon identifies genus-wide and species-specific targets of catabolite repression control in Pseudomonas bacteria.BMC microbiology · 2010Article
- Small RNA as global regulator of carbon catabolite repression in Pseudomonas aeruginosa.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Role of Acinetobacter baylyi Crc in catabolite repression of enzymes for aromatic compound catabolism.Journal of bacteriology · 2009Article
- The target for the Pseudomonas putida Crc global regulator in the benzoate degradation pathway is the BenR transcriptional regulator.Journal of bacteriology · 2008Article
- How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.Microbiology and molecular biology reviews : MMBR · 2006Review
- Role of the ptsN gene product in catabolite repression of the Pseudomonas putida TOL toluene degradation pathway in chemostat cultures.Applied and environmental microbiology · 2006Article
- Characterization of temporal protein production in Pseudomonas aeruginosa biofilms.Journal of bacteriology · 2005Article
- Adaptations of Pseudomonas aeruginosa to the cystic fibrosis lung environment can include deregulation of zwf, encoding glucose-6-phosphate dehydrogenase.Journal of bacteriology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The global regulatory protein Crc is involved in the repression of several catabolic pathways for sugars, hydrocarbons, and nitrogenated and aromatic compounds in Pseudomonas putida and Pseudomonas aeruginosa when other preferred carbon sources are present in the culture medium (catabolite repression), therefore modulating carbon metabolism. We have analyzed whether the levels or the activity of Crc is regulated. Crc activity was followed by its ability to inhibit the induction by alkanes of the P. putida OCT plasmid alkane degradation pathway when cells grow in a complete medium, where the effect of Crc is very strong. The abundance of crc transcripts and the amounts of Crc protein were higher under repressing conditions than under nonrepressing conditions. The presence of crc on a high-copy-number plasmid considerably increased Crc levels, but this impaired its ability to inhibit the alkane degradation pathway. Crc shows similarity to a family of nucleases that have highly conserved residues at their catalytic sites. Mutation of the corresponding residues in Crc (Asp220 and His246) led to proteins that can inhibit induction of the alkane degradation pathway when present at normal or elevated levels in the cell. Repression by these mutant proteins occurred only under repressing conditions. These results suggest that both the amounts and the activity of Crc are modulated and support previous proposals that Crc may form part of a signal transduction pathway. Furthermore, the activity of the mutant proteins suggests that Crc is not a nuclease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.