ArticlePLoS biology2005
Intravital observation of Plasmodium berghei sporozoite infection of the liver.
Article in PLoS biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 155 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
155 citing papers in PubMed.
- Tissue-Resident Macrophages: Linking Physiology and Inflammation in Infection.Clinical reviews in allergy & immunology · 2026Review
- The case for exploring innate memory in Plasmodium infection.Current opinion in microbiology · 2026Review
- Cas9-expressing HC-04 hepatocytes facilitate CRISPR-based analysis of Plasmodium falciparum sporozoite-host interactions.PLoS genetics · 2026Article
- Traction force generation in Plasmodium sporozoites is modulated by a surface adhesin.Journal of microscopy · 2026Article
- The Putative E3 Ubiquitin Ligase TEX1 Is Required for Nuclear Biology and Developmental Progression ofCells · 2026Article
- Bivalent virus-like particles expressing SPECT1 and CSP trigger pre-erythrocytic malaria immunity and protect against transgenicFrontiers in immunology · 2026Article
- A review on molecular markers ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Review
- Advances in human organs-on-chips and applications for drug screening and personalized medicine.Fundamental research · 2025Review
- The role of tissue oxygenation in obesity-related cardiometabolic complications.Reviews in endocrine & metabolic disorders · 2025Review
- Exploring the effects of gut microbiota on cholangiocarcinoma progression by patient-derived organoids.Journal of translational medicine · 2025Review
- Unbiased phage display screening identifies hidden malaria vaccine targets.Emerging microbes & infections · 2024Review
- TargetingVaccines · 2024Review
- Killing ofMicroorganisms · 2024Article
- Naturally acquired antibodies against Plasmodium vivax pre-erythrocytic stage vaccine antigens inhibit sporozoite invasion of human hepatocytes in vitro.Scientific reports · 2024Article
- Maternal nutrient metabolism in the liver during pregnancy.Frontiers in endocrinology · 2024Review
- Glycosylated nanoparticle-based PfCSP vaccine confers long-lasting antibody responses and sterile protection in mouse malaria model.NPJ vaccines · 2023Article
- Maternal hepatic immunology during pregnancy.Frontiers in immunology · 2023Review
- Ontogeny, functions and reprogramming of Kupffer cells upon infectious disease.Frontiers in immunology · 2023Review
- Hepatitis B Virus Targets Lipid Transport Pathways to Infect Hepatocytes.Cellular and molecular gastroenterology and hepatology · 2023Article
- The origins, isolation, and biological characterization of rodent malaria parasites.Parasitology international · 2022Review
95 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Plasmodium sporozoite invasion of liver cells has been an extremely elusive event to study. In the prevailing model, sporozoites enter the liver by passing through Kupffer cells, but this model was based solely on incidental observations in fixed specimens and on biochemical and physiological data. To obtain direct information on the dynamics of sporozoite infection of the liver, we infected live mice with red or green fluorescent Plasmodium berghei sporozoites and monitored their behavior using intravital microscopy. Digital recordings show that sporozoites entering a liver lobule abruptly adhere to the sinusoidal cell layer, suggesting a high-affinity interaction. They glide along the sinusoid, with or against the bloodstream, to a Kupffer cell, and, by slowly pushing through a constriction, traverse across the space of Disse. Once inside the liver parenchyma, sporozoites move rapidly for many minutes, traversing several hepatocytes, until ultimately settling within a final one. Migration damage to hepatocytes was confirmed in liver sections, revealing clusters of necrotic hepatocytes adjacent to structurally intact, sporozoite-infected hepatocytes, and by elevated serum alanine aminotransferase activity. In summary, malaria sporozoites bind tightly to the sinusoidal cell layer, cross Kupffer cells, and leave behind a trail of dead hepatocytes when migrating to their final destination in the liver.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.