ArticleMolecular and cellular biology2005
Biological and biochemical functions of RNA in the tetrahymena telomerase holoenzyme.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 34 citations in OpenAlex.
- In vivo architecture of the telomerase RNA catalytic core in Trypanosoma brucei.Nucleic acids research · 2021Article
- Composition and Function of Telomerase-A Polymerase Associated with the Origin of Eukaryotes.Biomolecules · 2020Review
- Mechanisms of template handling and pseudoknot folding in human telomerase and their manipulation to expand the sequence repertoire of processive repeat synthesis.Nucleic acids research · 2018Article
- Structure and folding of the Tetrahymena telomerase RNA pseudoknot.Nucleic acids research · 2017Article
- Structural basis of template-boundary definition in Tetrahymena telomerase.Nature structural & molecular biology · 2015Article
- Article
- Progress in structural studies of telomerase.Current opinion in structural biology · 2014Review
- A motif in the vertebrate telomerase N-terminal linker of TERT contributes to RNA binding and telomerase activity and processivity.Structure (London, England : 1993) · 2013Article
- Article
- New models of Tetrahymena telomerase RNA from experimentally derived constraints and modeling.Journal of the American Chemical Society · 2012Article
- Biogenesis of telomerase ribonucleoproteins.RNA (New York, N.Y.) · 2012Review
- Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65.Molecular cell · 2012Article
- Roles of telomerase reverse transcriptase N-terminal domain in assembly and activity of Tetrahymena telomerase holoenzyme.The Journal of biological chemistry · 2012Article
- Telomere lengthening and other functions of telomerase.Acta naturae · 2012Article
- Single-stranded DNA repeat synthesis by telomerase.Current opinion in chemical biology · 2011Review
- Telomerase: an RNP enzyme synthesizes DNA.Cold Spring Harbor perspectives in biology · 2011Review
- Ciliate telomerase RNA loop IV nucleotides promote hierarchical RNP assembly and holoenzyme stability.RNA (New York, N.Y.) · 2010Article
- An RPA-related sequence-specific DNA-binding subunit of telomerase holoenzyme is required for elongation processivity and telomere maintenance.Molecular cell · 2009Article
- Disease-associated human telomerase RNA variants show loss of function for telomere synthesis without dominant-negative interference.Molecular and cellular biology · 2008Article
- High-resolution physical and functional mapping of the template adjacent DNA binding site in catalytically active telomerase.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Telomerase extends chromosome ends by the synthesis of tandem simple-sequence repeats. Studies of minimal recombinant telomerase ribonucleoprotein (RNP) reconstituted in vitro have revealed sequences within the telomerase RNA subunit (TER) that are required to establish its internal template and other unique features of enzyme activity. Here we test the significance of these motifs following TER assembly into telomerase holoenzyme in vivo. We established a method for stable expression of epitope-tagged TER and TER variants in place of wild-type Tetrahymena TER. We found that sequence substitutions in nontemplate regions of TER altered telomere length maintenance in vivo, with an increase or decrease in the set point for telomere length homeostasis. We also characterized the in vitro activity of the telomerase holoenzymes reconstituted with TER variants, following RNA-based RNP affinity purification from cell extracts. We found that nontemplate sequence substitutions imposed specific defects in the fidelity and processivity of template use. These findings demonstrate nontemplate functions of TER that are critical for the telomerase holoenzyme catalytic cycle and for proper telomere length maintenance in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.