ArticleOncogene2005
Silencing of the Tropomyosin-1 gene by DNA methylation alters tumor suppressor function of TGF-beta.
Article in Oncogene, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 71 citations in OpenAlex.
- Tropomyosin1 isoforms underlie epithelial to mesenchymal plasticity, metastatic dissemination, and resistance to chemotherapy in high-grade serous ovarian cancer.Cell death and differentiation · 2024Article
- Roles of TGF β and FGF Signals in the Lens: Tropomyosin Regulation for Posterior Capsule Opacity.International journal of molecular sciences · 2018Review
- Tropomyosin 2 heterozygous knockout in mice using CRISPR-Cas9 system displays the inhibition of injury-induced epithelial-mesenchymal transition, and lens opacity.Mechanisms of ageing and development · 2018Article
- Loss of Tpm4.1 leads to disruption of cell-cell adhesions and invasive behavior in breast epithelial cells via increased Rac1 signaling.Oncotarget · 2017Article
- FGF2 antagonizes aberrant TGFβ regulation of tropomyosin: role for posterior capsule opacity.Journal of cellular and molecular medicine · 2017Article
- Inhibitors of DNA methylation support TGF-β1-inducedJournal of periodontal & implant science · 2017Article
- Tropomyosin-1 acts as a potential tumor suppressor in human oral squamous cell carcinoma.PloS one · 2017Article
- Epigenetic silencing of TPM2 contributes to colorectal cancer progression upon RhoA activation.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells.BMC cancer · 2015Article
- KSHV MicroRNAs Repress Tropomyosin 1 and Increase Anchorage-Independent Growth and Endothelial Tube Formation.PloS one · 2015Article
- miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-α activation.American journal of physiology. Renal physiology · 2013Article
- Top-down targeted proteomics for deep sequencing of tropomyosin isoforms.Journal of proteome research · 2013Article
- Elevated tropomyosin expression is associated with epithelial-mesenchymal transition of lens epithelial cells.Journal of cellular and molecular medicine · 2013Article
- miRNAs in breast cancer tumorigenesis (Review).Oncology reports · 2012Review
- Article
- FBXW7 influences murine intestinal homeostasis and cancer, targeting Notch, Jun, and DEK for degradation.The Journal of experimental medicine · 2011Article
- Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure.American journal of physiology. Cell physiology · 2010Article
- Mechanisms of the epithelial-mesenchymal transition by TGF-beta.Future oncology (London, England) · 2009Review
- Microarray-based cancer prediction using soft computing approach.Cancer informatics · 2009Article
- Multivariate gene expression analysis reveals functional connectivity changes between normal/tumoral prostates.BMC systems biology · 2008Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Loss of actin stress fibers has been associated with cell transformation and metastasis. TGF-beta induction of stress fibers in epithelial cells requires high molecular weight tropomyosins encoded by TPM1 and TPM2 genes. Here, we investigated the mechanism underlying the failure of TGF-beta to induce stress fibers and inhibit cell migration in metastatic cells. RT-PCR analysis in carcinoma cell lines revealed a significant reduction in TPM1 transcripts in metastatic MDA-MB-231, MDA-MB-435 and SW620 cell lines. Treatment of these cells with demethylating agent 5-aza-2'-deoxycytidine (5-aza-dC) increased mRNA levels of TPM1 with no effect on TPM2. Importantly, 5-aza-dC treatment of MDA-MB-231 cells restored TGF-beta induction of TPM1 and formation of stress fibers. Forced expression of TPM1 by using Tet-Off system increased stress fibers in MDA-MB-231 cells and reduced cell migration. A potential CpG island spanning the TPM1 proximal promoter, exon 1, and the beginning of intron 1 was identified. Bisulfite sequencing showed significant cytosine methylation in metastatic cell lines that correlated with a reduced expression of TPM1. Together these results suggest that epigenetic suppression of TPM1 may alter TGF-beta tumor suppressor function and contribute to metastatic properties of tumor cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.