ArticleProceedings of the National Academy of Sciences of the United States of America2005
Quantitating intracellular transport of polyplexes by spatio-temporal image correlation spectroscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 90 citations in OpenAlex.
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- Fluorogenic RNA-based biomaterials for imaging and tracking the cargo of extracellular vesicles.Journal of controlled release : official journal of the Controlled Release Society · 2024Article
- Spatiotemporal three-dimensional transport dynamics of endocytic cargos and their physical regulations in cells.iScience · 2022Article
- Insulin secretory granules labelled with phogrin-fluorescent proteins show alterations in size, mobility and responsiveness to glucose stimulation in living β-cells.Scientific reports · 2019Article
- Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles.Acta biomaterialia · 2016Article
- Intracellular kinetics of the androgen receptor shown by multimodal Image Correlation Spectroscopy (mICS).Scientific reports · 2016Article
- An Intermittent Model for Intracellular Motions of Gold Nanostars by k-Space Scattering Image Correlation.Biophysical journal · 2015Article
- Kinome-level screening identifies inhibition of polo-like kinase-1 (PLK1) as a target for enhancing non-viral transgene expression.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Insight into polycation chain length affecting transfection efficiency by O-methyl-free N,N,N-trimethyl chitosans as gene carriers.Pharmaceutical research · 2014Article
- Spatiotemporal cellular imaging of polymer-pDNA nanocomplexes affords in situ morphology and trafficking trends.Molecular pharmaceutics · 2013Article
- Quantitative measurement of intracellular transport of nanocarriers by spatio-temporal image correlation spectroscopy.Methods and applications in fluorescence · 2013Article
- Real-time gene delivery vector tracking in the endo-lysosomal pathway of live cells.Microscopy research and technique · 2012Article
- Characterization of dynamic actin associations with T-cell receptor microclusters in primary T cells.Journal of cell science · 2012Article
- Balancing protection and release of DNA: tools to address a bottleneck of non-viral gene delivery.Journal of the Royal Society, Interface · 2010Review
- Diffusive and directional intracellular dynamics measured by field-based dynamic light scattering.Optics express · 2010Article
- Lipophilic beta-cyclodextrin cyclic-nitrone conjugate: synthesis and spin trapping studies.The Journal of organic chemistry · 2009Article
- Microfabricated particles for engineered drug therapies: elucidation into the mechanisms of cellular internalization of PRINT particles.Pharmaceutical research · 2008Article
- Quantum dot-amphipol nanocomplex for intracellular delivery and real-time imaging of siRNA.ACS nano · 2008Article
- Differential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells.Genetic vaccines and therapy · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitatively understanding how nonviral gene delivery vectors (polyplexes) are transported inside cells is essential before they can be optimized for gene therapy and medical applications. In this study, we used spatio-temporal image correlation spectroscopy (ICS) to follow polymer-nucleic acid particles (polyplexes) of various sizes and analyze their diffusive-like and flow behaviors intracellularly to elucidate the mechanisms responsible for their transport. ICS is a quantitative imaging technique that allows the assessment of particle motion in complex systems, although it has not been widely used to date. We find that the internalized polyplexes are able to use microtubule motors for intracellular trafficking and exhibit different transport behaviors for short (<10 s) versus long (approximately 60 s) correlation times. This motion can be explained by a memory effect of the microtubule motors. These results reveal that, although microtubule motor biases may be present for short periods of time, resulting in a net directional velocity, the overall long-term motion of the polyplexes is best described as a random walk-like process. These studies suggest that spatio-temporal ICS is a powerful technique for assessing the nature of intracellular motion and provides a quantitative tool to compare the transport of different objects within a living cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.