ArticleNucleic acids research2005
Localization of spermine binding sites in 23S rRNA by photoaffinity labeling: parsing the spermine contribution to ribosomal 50S subunit functions.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Nanoparticle-Based mRNA Vaccine Induces Protective Neutralizing Antibodies Against Infectious Bronchitis Virus in In-Vivo Infection.Vaccines · 2025Article
- Polyamine Dysregulation and Nucleolar Disruption in Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2024Article
- Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism.Nucleic acids research · 2022Article
- An Epigenetics-Based Hypothesis of Autoantigen Development in Systemic Lupus Erythematosus.Epigenomes · 2020Review
- Analysis of crystalline and solution states of ligand-free spermidine N-acetyltransferase (SpeG) from Escherichia coli.Acta crystallographica. Section D, Structural biology · 2019Article
- Polyamines as Quality Control Metabolites Operating at the Post-Transcriptional Level.Plants (Basel, Switzerland) · 2019Review
- New Chloramphenicol Derivatives from the Viewpoint of Anticancer and Antimicrobial Activity.Antibiotics (Basel, Switzerland) · 2019Article
- Roles of polyamines in translation.The Journal of biological chemistry · 2018Review
- Polyamine Control of Translation Elongation Regulates Start Site Selection on Antizyme Inhibitor mRNA via Ribosome Queuing.Molecular cell · 2018Article
- Amino acid substrates impose polyamine, eIF5A, or hypusine requirement for peptide synthesis.Nucleic acids research · 2017Article
- Epigenetics and autoimmune diseases: the X chromosome-nucleolus nexus.Frontiers in genetics · 2015Article
- Endogenous polyamine function--the RNA perspective.Nucleic acids research · 2014Review
- Conjugation with polyamines enhances the antibacterial and anticancer activity of chloramphenicol.Nucleic acids research · 2014Article
- Article
- γ-glutamyl Spermine Synthetase PauA2 as a potential target of antibiotic development against Pseudomonas aeruginosa.Antimicrobial agents and chemotherapy · 2012Article
- Investigating the entire course of telithromycin binding to Escherichia coli ribosomes.Nucleic acids research · 2012Article
- Translational recoding as a feedback controller: systems approaches reveal polyamine-specific effects on the antizyme ribosomal frameshift.Nucleic acids research · 2011Article
- DNAzyme-mediated recovery of small recombinant RNAs from a 5S rRNA-derived chimera expressed in Escherichia coli.BMC biotechnology · 2010Article
- The effect of spermine on the initiation of mitochondrial protein synthesis.Biochemical and biophysical research communications · 2010Article
- Inhibition of antiassociation activity of translation initiation factor 3 by paromomycin.Antimicrobial agents and chemotherapy · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyamine binding to 23S rRNA was investigated, using a photoaffinity labeling approach. This was based on the covalent binding of a photoreactive analog of spermine, N1-azidobenzamidino (ABA)-spermine, to Escherichia coli ribosomes or naked 23S rRNA under mild irradiation conditions. The cross-linking sites of ABA-spermine in 23S rRNA were determined by RNase H digestion and primer-extension analysis. Domains I, II, IV and V in naked 23S rRNA were identified as discrete regions of preferred cross-linking. When 50S ribosomal subunits were targeted, the interaction of the photoprobe with the above 23S rRNA domains was elevated, except for helix H38 in domain II whose susceptibility to cross-linking was greatly reduced. In addition, cross-linking sites were identified in domains III and VI. Association of 30S with 50S subunits, poly(U), tRNA(Phe) and AcPhe-tRNA to form a post-translocation complex further altered the cross-linking, in particular to helices H11-H13, H21, H63, H80, H84, H90 and H97. Poly(U)-programmed 70S ribosomes, reconstituted from photolabeled 50S subunits and untreated 30S subunits, bound AcPhe-tRNA in a similar fashion to native ribosomes. However, they exhibited higher reactivity toward puromycin and enhanced tRNA-translocation efficiency. These results suggest an essential role for polyamines in the structural and functional integrity of the large ribosomal subunit.
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