ArticleParasitology research2005
Isolation of a heat-resistant allergen from the fish parasite Anisakis simplex.
Article in Parasitology research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Anisakiasis: A Decade of Molecular and Diagnostic Advancements (2015-2026).International journal of molecular sciences · 2026Review
- In Vitro Control of Anisakis Larvae Using Nanoemulsified Thyme, Garlic, and Grape Seed Essential Oils Combined with Brines.Acta parasitologica · 2025Article
- An Assessment of the Currently Available Molecular Assay for the Diagnosis of Anisakis Sensitization.International journal of molecular sciences · 2025Article
- Anisakidae and Anisakidosis: A Public Health Perspective.Pathogens (Basel, Switzerland) · 2025Review
- An investigation of the prevalence and diversity ofFrontiers in microbiology · 2024Article
- Article
- Correlation of NHR-48 Transcriptional Modulator Expression with Selected CYP Genes' Expression during Thiabendazole Treatment ofPathogens (Basel, Switzerland) · 2020Article
- Review
- Anisakis simplex products impair intestinal epithelial barrier function and occludin and zonula occludens-1 localisation in differentiated Caco-2 cells.PLoS neglected tropical diseases · 2020Article
- Article
- Potential Transfer of Peanut and/or Soy Proteins from Poultry Feed to the Meat and/or Eggs Produced.ACS omega · 2020Article
- Review
- Infective larvae ofBiology open · 2019Article
- Functional insights into the infective larval stage of Anisakis simplex s.s., Anisakis pegreffii and their hybrids based on gene expression patterns.BMC genomics · 2018Article
- Excretory/secretory products of anisakid nematodes: biological and pathological roles.Acta veterinaria Scandinavica · 2017Review
- Anisakis pegreffii (Nematoda: Anisakidae) products modulate oxidative stress and apoptosis-related biomarkers in human cell lines.Parasites & vectors · 2016Article
- The Anisakis Transcriptome Provides a Resource for Fundamental and Applied Studies on Allergy-Causing Parasites.PLoS neglected tropical diseases · 2016Article
- Characterisation of potential novel allergens in the fish parasiteEuPA open proteomics · 2014Article
- Allergic reactions to Anisakis found in fish.Current allergy and asthma reports · 2014Review
- Helminth Allergens, Parasite-Specific IgE, and Its Protective Role in Human Immunity.Frontiers in immunology · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The thermal stability of allergenic peptides from the fish parasite Anisakis simplex has not been fully elucidated. This is of special relevance for physicians who should clearly indicate if sensitized patients should avoid ingestion of raw fish only or whether well-cooked fish should also be avoided, if allergenic peptides derived from the parasite remain immunologically detectable. An allergen was purified after heating a crude parasite extract for 30 min. The allergen was further purified by an ethanol fractionation procedure followed by a reversed-phase HPLC. The N-terminal amino acid sequence was obtained. This allergen was detected by 27% of sensitized subjects. The N-terminal amino acid sequence of the 9 kDa allergen showed no similarities to other known proteins. A minor low molecular weight allergen from A. simplex is highly resistant to heating and it could therefore have significant clinical relevance.
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Registered trials
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