ArticleBiophysical journal2005
Measurement of single macromolecule orientation by total internal reflection fluorescence polarization microscopy.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 111 citations in OpenAlex.
- Torque-generating units of the bacterial flagellar motor are rotary motors.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Examination of conformational dynamics of AdiC transporter with fluorescence-polarization microscopy.The Journal of general physiology · 2025Article
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- Twist response of actin filaments.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Through the Eyes of Creators: Observing Artificial Molecular Motors.ACS nanoscience Au · 2022Review
- Angle change of the A-domain in a single SERCA1a molecule detected by defocused orientation imaging.Scientific reports · 2021Article
- Single-Molecule 3D Orientation Imaging Reveals Nanoscale Compositional Heterogeneity in Lipid Membranes.Angewandte Chemie (International ed. in English) · 2020Article
- Single-molecule polarization microscopy of DNA intercalators sheds light on the structure of S-DNA.Science advances · 2019Article
- Imaging the three-dimensional orientation and rotational mobility of fluorescent emitters using the Tri-spot point spread function.Applied physics letters · 2018Article
- Dimensions and Interactions of Large T-Cell Surface Proteins.Frontiers in immunology · 2018Review
- The Detection of Nanoscale Membrane Bending with Polarized Localization Microscopy.Biophysical journal · 2017Article
- Article
- Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking.Chemical reviews · 2017Review
- No Difference in Myosin Kinetics and Spatial Distribution of the Lever Arm in the Left and Right Ventricles of Human Hearts.Frontiers in physiology · 2017Article
- Single molecule optical measurements of orientation and rotations of biological macromolecules.Methods and applications in fluorescence · 2016Article
- Dissection of molecular assembly dynamics by tracking orientation and position of single molecules in live cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Internal and external components of the bacterial flagellar motor rotate as a unit.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Enhanced DNA imaging using super-resolution microscopy and simultaneous single-molecule orientation measurements.Optica · 2016Article
- Determining the rotational mobility of a single molecule from a single image: a numerical study.Optics express · 2015Article
- Single-molecule spectroscopy and imaging over the decades.Faraday discussions · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new approach is presented for measuring the three-dimensional orientation of individual macromolecules using single molecule fluorescence polarization (SMFP) microscopy. The technique uses the unique polarizations of evanescent waves generated by total internal reflection to excite the dipole moment of individual fluorophores. To evaluate the new SMFP technique, single molecule orientation measurements from sparsely labeled F-actin are compared to ensemble-averaged orientation data from similarly prepared densely labeled F-actin. Standard deviations of the SMFP measurements taken at 40 ms time intervals indicate that the uncertainty for individual measurements of axial and azimuthal angles is approximately 10 degrees at 40 ms time resolution. Comparison with ensemble data shows there are no substantial systematic errors associated with the single molecule measurements. In addition to evaluating the technique, the data also provide a new measurement of the torsional rigidity of F-actin. These measurements support the smaller of two values of the torsional rigidity of F-actin previously reported.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.