ArticleJournal of virology2005
vLIP, a viral lipase homologue, is a virulence factor of Marek's disease virus.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 75 citations in OpenAlex.
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- Characterizing a newly identified avian herpesvirus-specific gene SORF3 in DPV and its roles in potential pathogenicity.Journal of virology · 2026Article
- Pathogens That Rewrite the Rules: Ascoviruses, Elegant Manipulators of Cell Death Pathways and Architects of the Extracellular Viral Paradigm.Pathogens (Basel, Switzerland) · 2025Review
- Marek's disease virus-1 unique gene LORF1 is involved in viral replication and MDV-1/Md5-induced atrophy of the bursa of Fabricius.PLoS pathogens · 2025Article
- LORF9 of Marek's disease virus is involved in the early cytolytic replication of B lymphocytes and can act as a target for gene deletion vaccine development.Journal of virology · 2023Article
- Characterization of a Unique Novel LORF3 Protein of Duck Plague Virus and Its Potential Pathogenesis.Journal of virology · 2023Article
- Integrated viral elements suggest the dual lifestyle ofVirus evolution · 2022Article
- Latest Insights into Unique Open Reading Frames Encoded by Unique Long (UL) and Short (US) Regions of Marek's Disease Virus.Viruses · 2021Review
- Marek's Disease Virus Cluster 3 miRNAs Restrict Virus' Early Cytolytic Replication and Pathogenesis.Viruses · 2020Article
- Glutaminolysis and Glycolysis Are Essential for Optimal Replication of Marek's Disease Virus.Journal of virology · 2020Article
- Replication of Marek's Disease Virus Is Dependent on Synthesis ofJournal of virology · 2019Article
- An emerging new fowl adenovirus genotype.Heliyon · 2019Article
- The Transcriptional Landscape of Marek's Disease Virus in Primary Chicken B Cells Reveals Novel Splice Variants and Genes.Viruses · 2019Article
- Hydropericardium syndrome caused by fowl adenovirus serotype 4 in replacement pullets.The Journal of veterinary medical science · 2019Article
- The phylogenomics of evolving virus virulence.Nature reviews. Genetics · 2018Review
- Article
- A phylogenomic analysis of Marek's disease virus reveals independent paths to virulence in Eurasia and North America.Evolutionary applications · 2017Article
- Marek's disease in chickens: a review with focus on immunology.Veterinary research · 2016Review
- Virus and host genomic, molecular, and cellular interactions during Marek's disease pathogenesis and oncogenesis.Poultry science · 2016Review
- Identification of non-essential loci within the Meleagrid herpesvirus 1 genome.Virology journal · 2015Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of Marek's disease virus (MDV) has been predicted to encode a secreted glycoprotein, vLIP, which bears significant homology to the alpha/beta hydrolase fold of pancreatic lipases. Here it is demonstrated that MDV vLIP mRNA is produced via splicing and that vLIP is a late gene, due to its sensitivity to inhibition of DNA replication. While vLIP was found to conserve several residues essential to hydrolase activity, an unfavorable asparagine substitution is present at the lipase catalytic triad acid position. Consistent with structural predictions, purified recombinant vLIP did not show detectable activity on traditional phospholipid or triacylglyceride substrates. Two different vLIP mutant viruses, one bearing a 173-amino-acid deletion in the lipase homologous domain, the other having an alanine point mutant at the serine nucleophile position, caused a significantly lower incidence of Marek's disease in chickens and resulted in enhanced survival relative to two independently produced vLIP revertants or parental virus. These data provide the first evidence that vLIP enhances the replication and pathogenic potential of MDV. Furthermore, while vLIP may not serve as a traditional lipase enzyme, the data indicate that the serine nucleophile position is nonetheless essential in vivo for the viral functions of vLIP. Therefore, it is suggested that this particular example of lipase homology may represent the repurposing of an alpha/beta hydrolase fold toward a nonenzymatic role, possibly in lipid bonding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.