ArticleCellular microbiology2005
Trichomonas vaginalis adherence mediates differential gene expression in human vaginal epithelial cells.
Article in Cellular microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Trichomonosis and subsequent risk of prostate cancer in the Prostate Cancer Prevention Trial.International journal of cancer · 2009Trial
- Immunomodulatory Effects of the Antimicrobial Peptide KR-20: Implications for Trichomoniasis.Molecules (Basel, Switzerland) · 2026Article
- Interactions between bacterial vaginosis-associated microbiota and Trichomonas vaginalis modulate parasite-induced pathogenicity and host immune responses.Parasites & vectors · 2025Article
- Cas9 Contributes to Group B Streptococcal Colonization and Disease.Frontiers in microbiology · 2019Article
- Interaction betweenThe Korean journal of parasitology · 2017Article
- The Pathogenesis of Human Cervical Epithelium Cells Induced by Interacting with Trichomonas vaginalis.PloS one · 2015Article
- Trichonomas vaginalis metalloproteinase induces apoptosis of SiHa cells through disrupting the Mcl-1/Bim and Bcl-xL/Bim complexes.PloS one · 2014Article
- Biological roles of cysteine proteinases in the pathogenesis of Trichomonas vaginalis.Parasite (Paris, France) · 2014Review
- Comparative transcriptomic and proteomic analyses of Trichomonas vaginalis following adherence to fibronectin.Infection and immunity · 2012Article
- The Effect of Commiphora molmol (Myrrh) in Treatment of Trichomoniasis vaginalis infection.Iranian Red Crescent medical journal · 2011Article
- Impact of T. vaginalis infection on innate immune responses and reproductive outcome.Journal of reproductive immunology · 2009Review
- Genetic identity and differential gene expression between Trichomonas vaginalis and Trichomonas tenax.BMC microbiology · 2009Article
- Immunogenic and plasminogen-binding surface-associated alpha-enolase of Trichomonas vaginalis.Infection and immunity · 2008Article
- The proteins secreted by Trichomonas vaginalis and vaginal epithelial cell response to secreted and episomally expressed AP65.Cellular microbiology · 2007Article
- A novel surface protein of Trichomonas vaginalis is regulated independently by low iron and contact with vaginal epithelial cells.BMC microbiology · 2006Article
- Adherence to human vaginal epithelial cells signals for increased expression of Trichomonas vaginalis genes.Infection and immunity · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Trichomonas vaginalis, an ancient protist, colonizes the vaginal mucosa causing trichomonosis, a vaginitis that sometimes leads to severe health complications. Preparatory to colonization of the vagina is the adhesion to vaginal epithelial cells (VECs) by trichomonads. We hypothesized that VECs alter the gene expression to form a complex signalling cascade in response to trichomonal adherence. In order to identify the genes that are upregulated, we constructed a subtraction cDNA library after contact with parasites that is enriched for differentially expressed genes from the immortalized MS-74 VECs. Sixty cDNA clones were sequenced and to our knowledge for the first time, differentially regulated genes were identified in response to early trichomonal infection. The identified genes were found to encode functional proteins with specific functions associated with cell structure maintenance and extracellular matrix components, proinflammatory molecules and apoptosis. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) confirmed expression of selected genes. Further, cyclooxygenase 2 (COX-2) protein expression was analysed using Western blot and immunofluorescence assays. Data suggest that p38 mitogen-activated protein (MAP) kinase and tyrosine kinases play a role in COX-2 induction. Finally, T. vaginalis and Tritrichomonas foetus but not Pentatrichomonas hominis induce expression of COX-2. This is a first attempt at elucidating the basis of interaction of trichomonads with host cells and the corresponding host responses triggered by the parasites.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.