ArticleNucleic acids research2005
Molecular haplotyping by linking emulsion PCR: analysis of paraoxonase 1 haplotypes and phenotypes.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- High-throughput identification and quantification of single bacterial cells in the microbiota.Nature communications · 2022Article
- Prenatal maternal organophosphorus pesticide exposures, paraoxonase 1, and childhood adiposity in the Mount Sinai Children's Environmental Health Study.Environment international · 2020Article
- Uncovering microbial inter-domain interactions in complex communities.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Prenatal Organophosphorus Pesticide Exposure and Child Neurodevelopment at 24 Months: An Analysis of Four Birth Cohorts.Environmental health perspectives · 2016Article
- Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers.The ISME journal · 2016Article
- Multiplexed tracking of combinatorial genomic mutations in engineered cell populations.Nature biotechnology · 2015Article
- Haplotype-resolved genome sequencing: experimental methods and applications.Nature reviews. Genetics · 2015Review
- Determination of haplotypes at structurally complex regions using emulsion haplotype fusion PCR.BMC genomics · 2012Article
- Massively parallel haplotyping on microscopic beads for the high-throughput phase analysis of single molecules.PloS one · 2012Article
- Article
- Completely phased genome sequencing through chromosome sorting.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Experimental generation of SNP haplotype signatures in patients with sickle cell anaemia.PloS one · 2010Article
- A common polymorphism in the caspase recruitment domain of RIG-I modifies the innate immune response of human dendritic cells.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Search for genetic markers and functional variants involved in the development of opiate and cocaine addiction and treatment.Annals of the New York Academy of Sciences · 2010Review
- An emulsion system based on a chip polymerase chain reaction.Molecules (Basel, Switzerland) · 2008Article
- Paraoxonase polymorphisms, haplotypes, and enzyme activity in Latino mothers and newborns.Environmental health perspectives · 2006Article
- Assaying chromosomal inversions by single-molecule haplotyping.Nature methods · 2006Article
- Long-range multilocus haplotype phasing of the MHC.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Shotgun haplotyping: a novel method for surveying allelic sequence variation.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
Linking emulsion PCR (LE-PCR) enables formation of minichromosomes preserving phase information of two polymorphic loci, hence the haplotype. Emulsion PCR confines two amplicons of two linked polymorphic sites on a single template molecule to one aqueous-phase droplet. Linking PCR uses biotinylated, overlapping linking primers to connect these amplicons in the droplet. After LE-PCR, unlinked amplicons are removed on streptavidin-coated magnetic beads and single-stranded runoff products are capped by primer extension. Quantitative ASPCR can then be used to ascertain the haplotypes of the two polymorphic loci on the minichromosomes. Using LE-PCR, we determined the human paraoxonase-1 [PON1] molecular haplotypes at three loci (-909g>c, L55M, Q192R) in women who were compound heterozygotes for -909g>c/L55M (n = 89), -909g>c/Q192R (n = 77) and L55M/Q192R (n = 68). We observed a strong association between PON1 substrate specificity (paraoxon/phenylacetate substrate activity ratios) and -909g>c/Q192R haplotype. We have demonstrated here a powerful molecular haplotyping technology that can be applied in population studies.
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