ArticleAmerican journal of physiology. Renal physiology2005
Vasopressin increases urea permeability in the initial IMCD from diabetic rats.
Article in American journal of physiology. Renal physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Urea transport and clinical potential of urearetics.Current opinion in nephrology and hypertension · 2016Review
- Transgenic Restoration of Urea Transporter A1 Confers Maximal Urinary Concentration in the Absence of Urea Transporter A3.Journal of the American Society of Nephrology : JASN · 2016Article
- Glycosylation of solute carriers: mechanisms and functional consequences.Pflugers Archiv : European journal of physiology · 2016Review
- Activation of protein kinase C-α and Src kinase increases urea transporter A1 α-2, 6 sialylation.Journal of the American Society of Nephrology : JASN · 2015Article
- RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.Frontiers in physiology · 2015Article
- New advances in urea transporter UT-A1 membrane trafficking.International journal of molecular sciences · 2013Review
- Urine concentration in the diabetic mouse requires both urea and water transporters.American journal of physiology. Renal physiology · 2013Article
- Molecular mechanisms of urea transport in health and disease.Pflugers Archiv : European journal of physiology · 2012Review
- Glycoforms of UT-A3 urea transporter with poly-N-acetyllactosamine glycosylation have enhanced transport activity.American journal of physiology. Renal physiology · 2012Article
- Mature N-linked glycans facilitate UT-A1 urea transporter lipid raft compartmentalization.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Suppression subtractive hybridization analysis of low-protein diet- and vitamin D-induced gene expression from rat kidney inner medullary base.Physiological genomics · 2010Article
- Candesartan augments compensatory changes in medullary transport proteins in the diabetic rat kidney.American journal of physiology. Renal physiology · 2008Article
- Effect of Micardis on the expression of renal medulla aquaporin-2 in diabetic mice.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2008Article
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5 authors at 1 institution in 1 country.
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Abstract
In normal rats, vasopressin and hyperosmolality enhance urea permeability (P(urea)) in the terminal, but not in the initial inner medullary collecting duct (IMCD), a process thought to occur through the UT-A1 urea transporter. In the terminal IMCD, UT-A1 is detected as 97- and 117-kDa glycoproteins. However, in the initial IMCD, only the 97-kDa form is detected. During streptozotocin-induced diabetes mellitus, UT-A1 protein abundance is increased, and the 117-kDa UT-A1 glycoprotein appears in the initial IMCD. We hypothesize that the 117-kDa glycoprotein mediates the vasopressin- and osmolality-induced changes in P(urea). Thus, in the present study, we measured P(urea) in in vitro perfused initial IMCDs from diabetic rats by imposing a 5 mM bath-to-lumen urea gradient without any osmotic gradient. Basal P(urea) was similar in control vs. diabetic rats (3 +/- 1 vs. 5 +/- 1 x 10(-5) cm/s, n = 4, P = not significant). Vasopressin (10 nM) significantly increased P(urea) to 16 +/- 5 x 10(-5) cm/s (n = 4, P < 0.05) in diabetic but not in control rats. Forskolin (10 microM, adenylyl cyclase activator) also significantly increased P(urea) in diabetic rats. In contrast, increasing osmolality to 690 mosmol/kg H2O did not change P(urea) in diabetic rats. We conclude that initial IMCDs from diabetic rats have vasopressin- and forskolin-, but not hyperosmolality-stimulated P(urea). The appearance of vasopressin-stimulated P(urea) in initial IMCDs correlates with an increase in UT-A1 protein abundance and the appearance of the 117-kDa UT-A1 glycoprotein in this region during diabetes. This suggests that the 117-kDa UT-A1 glycoprotein is necessary for vasopressin-stimulated urea transport.
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