ArticleJournal of fluorescence2005
Optimization of gold nanoparticle-based DNA detection for microarrays.
Article in Journal of fluorescence, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed.
- A novel colorimetric DNA sensor for rapid detection of feline panleukopenia virus using ZnFeScientific reports · 2025Article
- PLLA Coating of Active Implants for Dual Drug Release.Molecules (Basel, Switzerland) · 2022Article
- Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells.International journal of nanomedicine · 2014Article
- Detection of target ssDNA using a microfabricated Hall magnetometer with correlated optical readout.Journal of biomedicine & biotechnology · 2012Article
- Biofunctionalization of zinc oxide nanowires for DNA sensory applications.Nanoscale research letters · 2011Article
- A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests.Sensors (Basel, Switzerland) · 2009Article
- Luminescent lanthanide nanoparticles as labels in DNA microarrays for quantification of methyl tertiary butyl ether degrading bacteria.Journal of nanoscience and nanotechnology · 2008Article
- A real-time PCR-based method for determining the surface coverage of thiol-capped oligonucleotides bound onto gold nanoparticles.Nucleic acids research · 2006Article
- Optical readout of gold nanoparticle-based DNA microarrays without silver enhancement.Biophysical journal · 2006Article
- The Use of Atomic Force Microscopy for 3D Analysis of Nucleic Acid Hybridization on Microarrays.Acta naturaeArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA microarrays are promising tools for fast and highly parallel DNA detection by means of fluorescence or gold nanoparticle labeling. However, substrate modification with silanes (as a prerequisite for capture DNA binding) often leads to inhomogeneous surfaces and/or nonspecific binding of the labeled DNA. We examined both different substrate cleaning and activating protocols and also different blocking strategies for optimizing the procedures, especially those for nanoparticle labeling. Contact angle measurements as well as fluorescence microscopy, atomic force microscopy (AFM), and a flatbed scanner were used to analyze the multiple-step process. Although the examined different cleaning and activating protocols resulted in considerably different contact angles, meaning different substrate wettability, silanization led to similar hydrophobic surfaces which could be revealed as smooth surfaces of about 2-4 nm roughness. The two examined silanes (3-glycidoxypropyltrimethoxysilane (GOPS) and 3-aminopropyltriethoxysilane (APTES)) differed in their DNA binding homogeneity, maximum signal intensities, and sensitivity. Nonspecific gold binding on APTES/PDC surfaces could be blocked by treatment in 3% bovine serum albumin (BSA).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.