ArticleProceedings of the National Academy of Sciences of the United States of America2005
Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 236 citations in OpenAlex.
- Elucidating carbohydrate preference and engineering glucose transport in Caldimonas thermodepolymerans for enhanced polyhydroxyalkanoate production.Applied microbiology and biotechnology · 2026Article
- A highly conserved ABC transporter mediates cello-oligosaccharide uptake in the extremely thermophilic, lignocellulolytic bacteriumApplied and environmental microbiology · 2026Article
- Maltodextrin transport in the extremely thermophilic, lignocellulose degrading bacteriumJournal of bacteriology · 2025Article
- Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota.Nature communications · 2024Article
- Sugar transport in thermophiles: Bridging lignocellulose deconstruction and bioconversion.Journal of industrial microbiology & biotechnology · 2024Review
- Effect of Free Cysteine Residues to Serine Mutation on Cellodextrin Phosphorylase.Journal of applied glycoscience · 2024Article
- Article
- Bacterial xylan utilization regulons: systems for coupling depolymerization of methylglucuronoxylans with assimilation and metabolism.Journal of industrial microbiology & biotechnology · 2022Article
- Biological cellulose saccharification using a coculture of Clostridium thermocellum and Thermobrachium celere strain A9.Applied microbiology and biotechnology · 2022Article
- Functional Analysis of HApplied and environmental microbiology · 2022Article
- Declining carbohydrate solubilization with increasing solids loading during fermentation of cellulosic feedstocks by Clostridium thermocellum: documentation and diagnostic tests.Biotechnology for biofuels and bioproducts · 2022Article
- Laboratory Evolution and Reverse Engineering ofApplied and environmental microbiology · 2021Article
- Short-Chain Cello-oligosaccharides: Intensification and Scale-up of Their Enzymatic Production and Selective Growth Promotion among Probiotic Bacteria.Journal of agricultural and food chemistry · 2020Article
- Importance ofMicroorganisms · 2020Article
- Pseudomonad reverse carbon catabolite repression, interspecies metabolite exchange, and consortial division of labor.Cellular and molecular life sciences : CMLS · 2020Review
- Bioaugmentation enhances dark fermentative hydrogen production in cultures exposed to short-term temperature fluctuations.Applied microbiology and biotechnology · 2020Article
- Development of a Genome-Scale Metabolic Model ofFrontiers in bioengineering and biotechnology · 2020Article
- Metabolism of sucrose in a non-fermentative Escherichia coli under oxygen limitation.Applied microbiology and biotechnology · 2019Article
- Dissecting cellobiose metabolic pathway and its application in biorefinery through consolidated bioprocessing inFungal biology and biotechnology · 2019Article
- Exploring the Relationship BetweenFrontiers in microbiology · 2019Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bioenergetics of cellulose utilization by Clostridium thermocellum was investigated. Cell yield and maintenance parameters, Y(X/ATP)True = 16.44 g cell/mol ATP and m = 3.27 mmol ATP/g cell per hour, were obtained from cellobiose-grown chemostats, and it was shown that one ATP is required per glucan transported. Experimentally determined values for G(ATP)P-T (ATP from phosphorolytic beta-glucan cleavage minus ATP for substrate transport, mol ATP/mol hexose) from chemostats fed beta-glucans with degree of polymerization (DP) 2-6 agreed well with the predicted value of (n-2)/n [corrected] (n = mean cellodextrin DP assimilated). A mean G(ATP)(P-T) value of 0.52 +/- 0.06 was calculated for cellulose-grown chemostat cultures, corresponding to n = 4.20 +/- 0.46. Determination of intracellular beta-glucan radioactivity resulting from 14C-labeled substrates showed that uptake is different for cellulose and cellobiose (G2). For 14C-cellobiose, radioactivity was greatest for G2; substantially smaller but measurable for G1, G3, and G4; undetectable for G5 and G6; and n was approximately 2. For 14C-cellulose, radioactivity was greatest for G5; lower but substantial for G6, G2, and G1; very low for G3 and G4; and n was approximately 4. These results indicate that: (i) C. thermocellum hydrolyzes cellulose by a different mode of action from the classical mechanism involving solubilization by cellobiohydrolase; (ii) bioenergetic benefits specific to growth on cellulose are realized, resulting from the efficiency of oligosaccharide uptake combined with intracellular phosphorolytic cleavage of beta-glucosidic bonds; and (iii) these benefits exceed the bioenergetic cost of cellulase synthesis, supporting the feasibility of anaerobic biotechnological processing of cellulosic biomass without added saccharolytic enzymes.
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