ArticleGenes & development2005
Overexpression of fasciculation and elongation protein zeta-1 (FEZ1) induces a post-entry block to retroviruses in cultured cells.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- FEZ1 Plays Dual Roles in Early HIV-1 Infection by Independently Regulating Capsid Transport and Host Interferon-Stimulated Gene Expression.Journal of virology · 2023Article
- FEZ1 phosphorylation regulates HSPA8 localization and interferon-stimulated gene expression.Cell reports · 2022Article
- Fasciculation and elongation zeta proteins 1 and 2: From structural flexibility to functional diversity.World journal of biological chemistry · 2019Review
- SUN2 Overexpression Deforms Nuclear Shape and Inhibits HIV.Journal of virology · 2016Article
- HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus.Nature communications · 2015Article
- Antiretroviral restriction factors.Current opinion in virology · 2011Review
- FEZ2 has acquired additional protein interaction partners relative to FEZ1: functional and evolutionary implications.PloS one · 2011Article
- Profile of Stephen P. Goff. Interview by Greg Williams.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- PDZD8 is a novel Gag-interacting factor that promotes retroviral infection.Journal of virology · 2010Article
- Controllable expansion of primary cardiomyocytes by reversible immortalization.Human gene therapy · 2009Article
- The brain-specific factor FEZ1 is a determinant of neuronal susceptibility to HIV-1 infection.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- HIV-1 exploits importin 7 to maximize nuclear import of its DNA genome.Retrovirology · 2009Article
- Article
- The Ezrin-radixin-moesin family member ezrin regulates stable microtubule formation and retroviral infection.Journal of virology · 2008Article
- Retroviral proteins that interact with the host cell cytoskeleton.Current opinion in immunology · 2007Review
- Isolation and characterization of human cells resistant to retrovirus infection.Retrovirology · 2007Article
- An intrinsic host defense against HIV-1 integration?The Journal of clinical investigation · 2007Article
- Moesin regulates stable microtubule formation and limits retroviral infection in cultured cells.The EMBO journal · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two mutant Rat2 fibroblast cell lines, R3-2 and R4-7, have been previously isolated by a selection for retrovirus resistance. We have now further analyzed the basis of the block to retroviral infection in the R3-2 line. Using Affymetrix GeneChip analysis, several genes were identified as differentially expressed in the mutant R3-2 line compared with the wild-type cells. One of the candidate gene products, FEZ1 (fasciculation and elongation protein zeta-1), a protein kinase C (PKC)zeta-interacting protein homologous to the Caenorhabditis elegans synaptic transport protein UNC-76, was found to be up-regulated >30-fold in the resistant R3-2 line. FEZ1 overexpression in Rat2 cells conferred a potent resistance to infection by genetically marked retroviruses, and the degree of retroviral resistance in both Rat2 fibroblasts and 293T cells tightly correlated with the expression level of FEZ1 transcripts. FEZ1-overexpressing Rat2 cells showed a similar phenotype to that of the mutant R3-2 line: Infection resulted in normal viral DNA synthesis but a reduction in the formation of circular DNA, indicating a block after reverse transcription but before nuclear entry. Partial knockdown of FEZ1 expression in R3-2 by RNA interference (RNAi) significantly reduced the resistance of this line to infection. Thus, our data suggest that FEZ1 overexpression is sufficient to explain the resistant phenotype of R3-2 cells and identify FEZ1 as a new gene capable of causing retrovirus resistance.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.