ArticleGenes & development2005
Specific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- The double life of mammalian DNA replication origins.Genes & development · 2025Review
- Review
- Genetic analysis of osteoblast activity identifies Zbtb40 as a regulator of osteoblast activity and bone mass.PLoS genetics · 2020Article
- Eukaryotic replication origins: Strength in flexibility.Nucleus (Austin, Tex.) · 2016Review
- Post-licensing Specification of Eukaryotic Replication Origins by Facilitated Mcm2-7 Sliding along DNA.Molecular cell · 2015Article
- Peaks cloaked in the mist: the landscape of mammalian replication origins.The Journal of cell biology · 2015Review
- The role of transcription in the activation of a Drosophila amplification origin.G3 (Bethesda, Md.) · 2014Article
- Allele-specific genome-wide profiling in human primary erythroblasts reveal replication program organization.PLoS genetics · 2014Article
- Integrative analysis of gene amplification in Drosophila follicle cells: parameters of origin activation and repression.Genes & development · 2011Article
- Bubble-chip analysis of human origin distributions demonstrates on a genomic scale significant clustering into zones and significant association with transcription.Genome research · 2011Article
- Eukaryotic DNA replication origins: many choices for appropriate answers.Nature reviews. Molecular cell biology · 2010Review
- Heterochromatin protein 1 (HP1) modulates replication timing of the Drosophila genome.Genome research · 2010Article
- A winding road to origin discovery.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Transcription initiation activity sets replication origin efficiency in mammalian cells.PLoS genetics · 2009Article
- A revisionist replicon model for higher eukaryotic genomes.Journal of cellular biochemistry · 2008Review
- Overreplication of short DNA regions during S phase in human cells.Genes & development · 2008Article
- A miR-24 microRNA binding-site polymorphism in dihydrofolate reductase gene leads to methotrexate resistance.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Discrete functional elements required for initiation activity of the Chinese hamster dihydrofolate reductase origin beta at ectopic chromosomal sites.Experimental cell research · 2007Article
- Binding of AlF-C, an Orc1-binding transcriptional regulator, enhances replicator activity of the rat aldolase B origin.Molecular and cellular biology · 2006Article
- Genome-wide mapping of ORC and Mcm2p binding sites on tiling arrays and identification of essential ARS consensus sequences in S. cerevisiae.BMC genomics · 2006Article
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2 authors.
Funding
Abstract
The Chinese hamster dihydrofolate reductase (DHFR) origin of replication consists of a 55-kb zone of potential initiation sites lying between the convergently transcribed DHFR and 2BE2121 genes. Two subregions within this zone (ori-beta/ori-beta' and ori-gamma) are preferred. In the DHFR-deficient variant, DR8, which has deleted a 14-kb sequence straddling the 3' end of the DHFR gene, early-firing origin activity in the downstream ori-beta/ori-beta' and ori-gamma regions is completely suppressed. We show that the critical deleted sequences reside within a 168-bp segment encompassing the intron 5/exon 6 boundary, exon 6, 54 bp of the 3' untranslated region (UTR), but not the three natural polyA sites. In wild-type cells, this sequence efficiently arrests transcription in a region a few kilobases downstream, which coincides with the 5' boundary of the replication initiation zone. In DR8, DHFR-specific transcripts efficiently use an alternative sixth exon (6c) and polyA signals near the middle of the former intergenic region to process primary transcripts. However, transcription proceeds to a position almost 35 kb downstream from these signals, and replication initiation can only be detected beyond this point. When the wild-type 168-bp 3' element is inserted into DR8 at the same position as alternative exon 6c, transcription is arrested efficiently and initiations occur almost immediately downstream. Thus, the normal 3' end of the DHFR gene constitutes a boundary element not only for the gene but also for the local origin of replication.
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