ArticleEukaryotic cell2005
Developmental variation in Rab11-dependent trafficking in Trypanosoma brucei.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 67 citations in OpenAlex.
- The actomyosin system is essential for the integrity of the endosomal system in bloodstream formeLife · 2024Article
- Continuous endosomes form functional subdomains and orchestrate rapid membrane trafficking in trypanosomes.eLife · 2024Article
- Characterisation of TbSmee1 suggests endocytosis allows surface-bound cargo to enter the trypanosome flagellar pocket.Journal of cell science · 2023Article
- TbKINX1B: a novel BILBO1 partner and an essential protein in bloodstream form Trypanosoma brucei.Parasite (Paris, France) · 2022Article
- To the Surface and Back: Exo- and Endocytic Pathways inFrontiers in cell and developmental biology · 2021Review
- Late ESCRT machinery mediates the recycling and Rescue of Invariant Surface Glycoprotein 65 in Trypanosoma brucei.Cellular microbiology · 2020Article
- The Trypanosomal Transferrin Receptor of Trypanosoma Brucei-A Review.Tropical medicine and infectious disease · 2019Review
- Review
- Rab11 mediates selective recycling and endocytic trafficking in Trypanosoma brucei.Traffic (Copenhagen, Denmark) · 2018Article
- Surface proteins, ERAD and antigenic variation in Trypanosoma brucei.Cellular microbiology · 2016Article
- Transferrin: Endocytosis and Cell Signaling in Parasitic Protozoa.BioMed research international · 2015Review
- Iron Homeostasis and Trypanosoma brucei Associated Immunopathogenicity Development: A Battle/Quest for Iron.BioMed research international · 2015Review
- Life and times: synthesis, trafficking, and evolution of VSG.Trends in parasitology · 2014Review
- The ancient small GTPase Rab21 functions in intermediate endocytic steps in trypanosomes.Eukaryotic cell · 2014Article
- Acidocalcisome is required for autophagy in Trypanosoma brucei.Autophagy · 2014Article
- Characterization of the late endosomal ESCRT machinery in Trypanosoma brucei.Traffic (Copenhagen, Denmark) · 2013Article
- TbFRP, a novel FYVE-domain containing phosphoinositide-binding Ras-like GTPase from trypanosomes.Experimental parasitology · 2013Article
- Evidence for recycling of invariant surface transmembrane domain proteins in African trypanosomes.Eukaryotic cell · 2013Article
- Article
- Affinity is an important determinant of the anti-trypanosome activity of nanobodies.PLoS neglected tropical diseases · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Trypanosoma brucei, endocytosis is developmentally regulated and is substantially more active in the mammalian infective stage, where it likely plays a role in immune evasion. The small GTPase TbRAB11 is highly expressed in the mammalian stage and mediates recycling of glycosylphosphatidylinositol-anchored proteins, including the variant surface glycoprotein (VSG) and the transferrin receptor, plus trafficking of internalized anti-VSG antibody and transferrin. No function has been assigned to TbRAB11 in the procyclic (insect) stage trypanosome. The importance of TbRAB11 to both bloodstream and procyclic form viability was assessed by RNA interference (RNAi). Suppression of TbRAB11 in the bloodstream form was rapidly lethal and led to cells with round morphology and an enlarged flagellar pocket. TbRAB11 RNAi was also lethal in procyclic forms, which also became rounded, but progression to cell death was significantly slower and the flagellar pocket remained normal. In bloodstream forms, silencing of TbRAB11 had no effect on exocytosis of newly synthesized VSG, fluid-phase endocytosis, or transferrin uptake, but export of internalized transferrin was inhibited. Lectin endocytosis assays revealed a block to postendosomal transport mediated by suppressing TbRAB11. By contrast, in procyclic forms, depletion of TbRAB11 blocks both fluid-phase endocytosis and internalization of surface proteins. In normal bloodstream forms, most VSG is recycled, but in procyclics, internalized surface proteins accumulated in the lysosome. These data demonstrate that TbRAB11 controls recycling and is essential in both life stages of T. brucei but that its primary role is subject to developmental variation.
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