ArticleEukaryotic cell2005
Relationship of DFG16 to the Rim101p pH response pathway in Saccharomyces cerevisiae and Candida albicans.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 87 citations in OpenAlex.
- Molecular Triggers of Yeast Pathogenicity in the Yeast-Host Interactions.Current issues in molecular biology · 2025Review
- Transcriptional expression ofMicrobiology spectrum · 2024Article
- Candida albicans Hyphal Morphogenesis within Macrophages Does Not Require Carbon Dioxide or pH-Sensing Pathways.Infection and immunity · 2023Article
- Developing novel antifungals: lessons from G protein-coupled receptors.Trends in pharmacological sciences · 2023Review
- Conserved and Divergent Features of pH Sensing in Major Fungal Pathogens.Current clinical microbiology reports · 2023Review
- A Genome-Wide Screen for Genes Affecting Spontaneous Direct-Repeat Recombination inG3 (Bethesda, Md.) · 2020Article
- Transcriptional control of hyphal morphogenesis in Candida albicans.FEMS yeast research · 2020Review
- Aggregate Filamentous Growth Responses in Yeast.mSphere · 2019Article
- Yeast Cells Exposed to Exogenous Palmitoleic Acid Either Adapt to Stress and Survive or Commit to Regulated Liponecrosis and Die.Oxidative medicine and cellular longevity · 2018Review
- Article
- Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein.Molecular microbiology · 2015Article
- The Cryptococcus neoformans alkaline response pathway: identification of a novel rim pathway activator.PLoS genetics · 2015Article
- Hyphal growth in Candida albicans does not require induction of hyphal-specific gene expression.Molecular biology of the cell · 2015Article
- Signaling events of the Rim101 pathway occur at the plasma membrane in a ubiquitination-dependent manner.Molecular and cellular biology · 2014Article
- pH signaling in human fungal pathogens: a new target for antifungal strategies.Eukaryotic cell · 2014Review
- Mechanism of liponecrosis, a distinct mode of programmed cell death.Cell cycle (Georgetown, Tex.) · 2014Article
- Physical and genetic-interaction density reveals functional organization and informs significance cutoffs in genome-wide screens.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Variance heterogeneity in Saccharomyces cerevisiae expression data: trans-regulation and epistasis.PloS one · 2013Article
- Dynamic transcript profiling of Candida albicans infection in zebrafish: a pathogen-host interaction study.PloS one · 2013Article
- Membrane protein Rim21 plays a central role in sensing ambient pH in Saccharomyces cerevisiae.The Journal of biological chemistry · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Many fungal pH responses depend upon conserved Rim101p/PacC transcription factors, which are activated by C-terminal proteolytic processing. The means by which environmental pH is sensed by this pathway are not known. Here, we report a screen of the Saccharomyces cerevisiae viable deletion mutant library that has yielded a new gene required for processed Rim101p accumulation, DFG16. An S. cerevisiae dfg16Delta mutant expresses Rim101p-repressed genes at elevated levels. In addition, Candida albicans dfg16Delta/dfg16Delta mutants are defective in alkaline pH-induced filamentation, and their defect is suppressed by expression of truncated Rim101-405p. Thus, Dfg16p is a functionally conserved Rim101p pathway member. Many proteins required for processed Rim101p accumulation are members of the ESCRT complex, which functions in the formation of multivesicular bodies (MVBs). Staining with the dye FM4-64 indicates that the S. cerevisiae dfg16Delta mutant does not have an MVB defect. We find that two transcripts, PRY1 and ASN1, respond to mutations that affect both the Rim101p and MVB pathways but not to mutations that affect only one pathway. The S. cerevisiae dfg16Delta mutation does not affect PRY1 and ASN1 expression, thus confirming that Dfg16p function is restricted to the Rim101p pathway. Dfg16p is homologous to Aspergillus nidulans PalH, a component of the well-characterized PacC processing pathway. We verify that the previously recognized PalH homolog, Rim21p, also functions in the S. cerevisiae Rim101p pathway. Dfg16p is predicted to have seven membrane-spanning segments and a long hydrophilic C-terminal region, as expected if Dfg16p were a G-protein-coupled receptor.
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