ArticleEukaryotic cell2005
Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 51 citations in OpenAlex.
- Orchestrating homolog segregation in meiosis I: molecular logic and regulatory networks with emphasis on male metaphase I.Cell communication and signaling : CCS · 2026Review
- Spindle integrity is regulated by a phospho-dependent interaction between the Ndc80 and Dam1 kinetochore complexes.PLoS genetics · 2025Article
- Distinctive Growth and Transcriptional Changes of the DiatomFrontiers in microbiology · 2020Article
- Genome Wide Analysis of WD40 Proteins in Saccharomyces cerevisiae and Their Orthologs in Candida albicans.The protein journal · 2019Article
- M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s.Cell division · 2019Article
- Implications of alternative routes to APC/C inhibition by the mitotic checkpoint complex.PLoS computational biology · 2018Article
- The kinetochore-dependent and -independent formation of the CDC20-MAD2 complex and its functions in HeLa cells.Scientific reports · 2017Article
- Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions.G3 (Bethesda, Md.) · 2016Article
- The mitotic checkpoint complex (MCC): looking back and forth after 15 years.AIMS molecular science · 2016Article
- Connecting the microtubule attachment status of each kinetochore to cell cycle arrest through the spindle assembly checkpoint.Chromosoma · 2015Review
- Whole-exome sequencing reveals the mutational spectrum of testicular germ cell tumours.Nature communications · 2015Article
- Article
- Chromosome segregation in budding yeast: sister chromatid cohesion and related mechanisms.Genetics · 2014Review
- Determinants of robustness in spindle assembly checkpoint signalling.Nature cell biology · 2013Article
- Kinetochore localized Mad2 and Cdc20 is itself insufficient for triggering the mitotic checkpoint when Mps1 is low in Drosophila melanogaster neuroblasts.Cell cycle (Georgetown, Tex.) · 2012Article
- A quantitative model of the initiation of DNA replication in Saccharomyces cerevisiae predicts the effects of system perturbations.BMC systems biology · 2012Article
- Checkpoint-independent stabilization of kinetochore-microtubule attachments by Mad2 in human cells.Current biology : CB · 2012Article
- Mad2 and Mad3 cooperate to arrest budding yeast in mitosis.Current biology : CB · 2012Article
- General aspects of colorectal cancer.ISRN oncology · 2012Article
- Spindle assembly checkpoint: the third decade.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Favored models of spindle checkpoint signaling propose that two inhibitory complexes (Mad2-Cdc20 and Mad2-Mad3-Bub3-Cdc20) must be assembled at kinetochores in order to inhibit mitosis. We have directly tested this model in the budding yeast Saccharomyces cerevisiae. The proteins Mad2, Mad3, Bub3, Cdc20, and Cdc27 in yeast were quantified, and there are sufficient amounts to form stoichiometric inhibitors of Cdc20 and the anaphase-promoting complex. Mad2 is present in two separate complexes in cells arrested in mitosis with nocodazole. There is a small amount of Mad2-Mad3-Bub3-Cdc20 and a much larger amount of a complex that contains Mad2-Cdc20. We use conditional mutants to show that both Mad2 and Mad3 are essential for establishment and maintenance of the spindle checkpoint. Both spindle checkpoint complexes containing Mad2 form in mitosis, not in response to checkpoint activation. The kinetochore is not required to form either complex. We propose that the conversion of Mad1-Mad2 to Cdc20-Mad2, a key step in generating inhibitory checkpoint complexes, is limited to mitosis by the availability of Cdc20 and is kinetochore independent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.