ArticleBiophysical journal2005
Large-scale quantitative analysis of sources of variation in the actin polymerization-based movement of Listeria monocytogenes.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Doppler imaging detects bacterial infection of living tissue.Communications biology · 2021Article
- Article
- Cell and tissue morphology determine actin-dependent nuclear migration mechanisms in neuroepithelia.The Journal of cell biology · 2019Article
- Article
- Control of actin-based motility through localized actin binding.Physical biology · 2013Article
- Actin filament curvature biases branching direction.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Choosing orientation: influence of cargo geometry and ActA polarization on actin comet tails.Molecular biology of the cell · 2012Article
- A model actin comet tail disassembling by severing.Physical biology · 2011Article
- A microscopic formulation for the actin-driven motion of listeria in curved paths.Biophysical journal · 2010Article
- Bipedal locomotion in crawling cells.Biophysical journal · 2010Article
- An experimental and computational study of the effect of ActA polarity on the speed of Listeria monocytogenes actin-based motility.PLoS computational biology · 2009Article
- Models for actin polymerization motors.Journal of mathematical biology · 2009Review
- Article
- A kinematic description of the trajectories of Listeria monocytogenes propelled by actin comet tails.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Mechanics and dynamics of actin-driven thin membrane protrusions.Biophysical journal · 2006Article
- Adhesion controls bacterial actin polymerization-based movement.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Bacterial shape and ActA distribution affect initiation of Listeria monocytogenes actin-based motility.Biophysical journal · 2005Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
During the actin polymerization-based movement of Listeria monocytogenes, individual bacteria are rapidly propelled through the host cell cytoplasm by the growth of a filamentous actin tail. The rate of propulsion varies significantly among individuals and over time. To study this variation, we used a high-throughput tracking technique to record the movement of a large number (approximately 7900) of bacteria in Xenopus frog egg extract. Most bacteria (70%) appeared to maintain an individual characteristic speed over several minutes, suggesting that the major source of variation in average speed is intrinsic to the bacterium. Thirty percent of bacteria had significant changes in speed over time spans of a few minutes, including 17% that appeared to collide with obstacles and 13% that moved with a significant periodic component. For the latter, the peak frequency was proportional to speed, suggesting a mechanism with a fixed spatial scale of approximately 0.6 bacterial length. Near the rear of the bacterium, temporal fluctuations in actin density were positively correlated with fluctuations in speed, whereas near the front the correlation was negative. A comparison of the performance of linear models that predict motion given actin density suggests that the mechanism has a history of 5-10 s, and that fluctuations in actin density near the front of the bacteria contain more predictive information than the rear. Our results are consistent with physical models where bacterial speed is governed by the rate of dissociation of bonds between the bacterial surface and the actin tail, and individual variation is determined by long-lived intrinsic variability in bacterial surface properties.
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