ArticleBiophysical journal2005
Local mobility in lipid domains of supported bilayers characterized by atomic force microscopy and fluorescence correlation spectroscopy.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
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- A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control.Nature methods · 2024Article
- Fluorophore position of headgroup-labeled GbBiophysical journal · 2023Article
- Biophysical studies of lipid nanodomains using different physical characterization techniques.Biophysical journal · 2023Review
- Imaging non-classical mechanical responses of lipid membranes using molecular rotors.Chemical science · 2020Article
- Hetero-multivalent binding of cholera toxin subunit B with glycolipid mixtures.Colloids and surfaces. B, Biointerfaces · 2017Article
- GM1 Softens POPC Membranes and Induces the Formation of Micron-Sized Domains.Biophysical journal · 2016Article
- Filming biomolecular processes by high-speed atomic force microscopy.Chemical reviews · 2014Review
- Ganglioside influence on phospholipid films investigated with single molecule fluorescence measurements.The journal of physical chemistry. B · 2013Article
- Lipid Phase Separation and Protein-Ganglioside Clustering in Supported Bilayers Are Induced by Photorelease of Ceramide.Soft matter · 2013Article
- Protein-phospholipid interactions in nonclassical protein secretion: problem and methods of study.International journal of molecular sciences · 2013Article
- Receptor concentration and diffusivity control multivalent binding of Sv40 to membrane bilayers.PLoS computational biology · 2013Article
- Single plasmonic nanoparticle tracking studies of solid supported bilayers with ganglioside lipids.Journal of the American Chemical Society · 2012Article
- Single molecule probes of membrane structure: orientation of BODIPY probes in DPPC as a function of probe structure.The Analyst · 2012Article
- STED nanoscopy reveals molecular details of cholesterol- and cytoskeleton-modulated lipid interactions in living cells.Biophysical journal · 2011Article
- Lipid-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2011Review
- Super-resolution microscopy of lipid bilayer phases.Journal of the American Chemical Society · 2011Article
- Material properties of lipid microdomains: force-volume imaging study of the effect of cholesterol on lipid microdomain rigidity.Biophysical journal · 2010Article
- Heterogeneous diffusion of a membrane-bound pHLIP peptide.Biophysical journal · 2010Article
- Recent developments in fluorescence correlation spectroscopy for diffusion measurements in planar lipid membranes.International journal of molecular sciences · 2010Review
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3 authors.
Funding
Abstract
Fluorescence correlation spectroscopy (FCS) is used to examine mobility of labeled probes at specific sites in supported bilayers consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipid domains in 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). Those sites are mapped beforehand with simultaneous atomic force microscopy and submicron confocal fluorescence imaging, allowing characterization of probe partitioning between gel DPPC and disordered liquid DOPC domains with corresponding topography of domain structure. We thus examine the relative partitioning and mobility in gel and disordered liquid phases for headgroup- and tailgroup-labeled GM1 ganglioside probes and for headgroup- and tailgroup-labeled phospholipid probes. For the GM1 probes, large differences in mobility between fluid and gel domains are observed; whereas unexpected mobility is observed in submicron gel domains for the phospholipid probes. We attribute the latter to domain heterogeneities that could be induced by the probe. Furthermore, fits to the FCS data for the phospholipid probes in the DOPC fluid phase require two components (fast and slow). Although proximity to the glass substrate may be a factor, local distortion of the probe by the fluorophore could also be important. Overall, we observe nonideal aspects of phospholipid probe mobility and partitioning that may not be restricted to supported bilayers.
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