ArticleNucleic acids research2005
Production of viral vectors using recombinase-mediated cassette exchange.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 18 citations in OpenAlex.
- Preferable sites and orientations of transgene inserted in the adenovirus vector genome: The E3 site may be unfavorable for transgene position.Gene therapy · 2015Article
- Enhancing the bystander killing effect of an oncolytic HSV by arming it with a secretable apoptosis activator.Gene therapy · 2015Article
- Adenovirus-encoding virus-associated RNAs suppress HDGF gene expression to support efficient viral replication.PloS one · 2014Article
- Efficient production of adenovirus vector lacking genes of virus-associated RNAs that disturb cellular RNAi machinery.Scientific reports · 2013Article
- High-level expression by tissue/cancer-specific promoter with strict specificity using a single-adenoviral vector.Nucleic acids research · 2011Article
- A novel and simple method for construction of recombinant adenoviruses.Nucleic acids research · 2006Article
- Recommended Method for Chromosome Exploitation: RMCE-based Cassette-exchange Systems in Animal Cell Biotechnology.Cytotechnology · 2006Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA viruses are often used as vectors for foreign gene expression, but large DNA region from cloned or authentic viral genomes must usually be handled to generate viral vectors. Here, we present a unique system for generating adenoviral vectors by directly substituting a gene of interest in a small transfected plasmid with a replaced gene in a replicating viral genome in Cre-expressing 293 cells using the recombinase-mediated cassette exchange (RMCE) reaction. In combination with a positive selection of the viral cis-acting packaging signal connected with the gene of interest, the purpose vector was enriched to 97.5 and 99.8% after three and four cycles of infection, respectively. Our results also showed that the mutant loxP V (previously called loxP 2272), a variant target of Cre used in the RMCE reaction, was useful as a non-compatible mutant to wild-type loxP. This method could be useful for generating not only a large number of adenovirus vectors simultaneously, but also other DNA virus vectors including helper-dependent adenovirus vector.
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Registered trials
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