Evidence map›Paper›PMID 15879035›Full record

ArticleClinical and diagnostic laboratory immunology2005

Construction and expression of recombinant streptolysin-o and preevaluation of its use in immunoassays.

Blanca Velázquez, Hugo Massaldi, Julio Battistoni, José A Chabalgoity

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Clinical and diagnostic laboratory immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Blanca VelázquezDepartment of Biotechnology, Institute of Hygiene, School of Medicine, Universidad de la República, Uruguay, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
Hugo Massaldi
Julio Battistoni
José A Chabalgoity

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Commercially available immunoassays for assessment of anti-streptolysin-O antibodies use native streptolysin-O obtained by a complex process. We prepared a biologically active recombinant streptolysin-O with higher yield and a simpler purification process. An enzyme-linked immunosorbent assay developed with this recombinant showed good correlation with a commercial test, suggesting that it could be suitable for immunoassays.

Indexed as

Antibodies, BacterialBacterial ProteinsChromatography, AffinityCloning, MolecularEnzyme-Linked Immunosorbent AssayGlutathione TransferaseHumansPolymerase Chain ReactionRecombinant Fusion ProteinsStreptococcal InfectionsStreptococcus pyogenesStreptolysinsAntibodies, BacterialBacterial ProteinsGlutathione TransferaseRecombinant Fusion Proteinsstreptolysin OStreptolysins

Identifiers

PMID15879035
PMCPMC1112085

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.