ArticleClinical and diagnostic laboratory immunology2005
Comparison of a new immunochromatographic test to enzyme-linked immunosorbent assay for rapid detection of immunoglobulin m antibodies to hepatitis e virus in human sera.
Article in Clinical and diagnostic laboratory immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Point-of-Care Testing for Hepatitis Viruses: A Growing Need.Life (Basel, Switzerland) · 2023Review
- First serological and molecular investigation of hepatitis E virus infection in dromedary camels in Algeria.Frontiers in veterinary science · 2023Article
- Laboratory Diagnosis of HEV Infection.Advances in experimental medicine and biology · 2023Review
- Advances in Hepatitis E Virus Biology and Pathogenesis.Viruses · 2021Review
- Implications of Hepatitis E Virus in Blood Transfusions, Hemodialysis, and Solid Organ Transplants.Medicina (Kaunas, Lithuania) · 2020Review
- Article
- Epidemiology of zoonotic hepatitis E: a community-based surveillance study in a rural population in China.PloS one · 2014Article
- Hepatitis E virus infection.Clinical microbiology reviews · 2014Review
- Diagnosis of hepatitis E.Nature reviews. Gastroenterology & hepatology · 2013Review
- Seroprevalence study in forestry workers from eastern Germany using novel genotype 3- and rat hepatitis E virus-specific immunoglobulin G ELISAs.Medical microbiology and immunology · 2012Article
- Profile of acute infectious markers in sporadic hepatitis E.PloS one · 2010Article
- Application of truncated immunodominant polypeptide from hepatitis E virus (HEV) ORF2 in an assay to exclude nonspecific binding in detecting anti-HEV immunoglobulin M.Journal of clinical microbiology · 2010Article
- Double-antigen enzyme-linked immunosorbent assay for detection of hepatitis E virus-specific antibodies in human or swine sera.Clinical and vaccine immunology : CVI · 2008Article
- Characterization of hepatitis E-specific cell-mediated immune response using IFN-gamma ELISPOT assay.Journal of immunological methods · 2007Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An immunochromatographic test for rapid detection of IgM antibodies in patients with acute hepatitis E infection was developed utilizing the well-characterized recombinant protein EP2.1 and monoclonal antibody 4B2. The new rapid test based on a novel reverse-flow technology was able to generate a positive result within 2 to 3 min. Our study showed that this test was able to detect anti-HEV IgM antibodies in 96.7% of the patient samples tested (n = 151) while maintaining an excellent specificity of 98.6% with samples from various patient or healthy control groups (total n = 208). Furthermore, this rapid test gave a good specificity of 90.9% when tested with rheumatoid factor (RF)-positive sera (RF value of < or =850 IU/ml; n = 11) although a higher concentration of RF in samples might cause cross-reactivity. The new test has a good agreement of 97.2% with a kappa value of 0.943 when compared with a reference enzyme-linked immunosorbent assay. The positive predictive value and the negative predictive value for the rapid test thus reached 98.0 and 97.6%, respectively. This is the first rapid, point-of-care test for hepatitis E and will be especially useful for the diagnosis of acute hepatitis E virus infection in field and emergency settings and in resource-poor countries.
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