ArticleJournal of clinical microbiology2005
Comparison of six methods of extracting Mycobacterium tuberculosis DNA from processed sputum for testing by quantitative real-time PCR.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 147 citations in OpenAlex.
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- Biofilm production and virulence traits among extensively drug-resistant and methicillin-resistant Staphylococcus aureus from buffalo subclinical mastitis in Bangladesh.Scientific reports · 2025Article
- Whole-genome sequencing for analyzing the transmission characteristics of drug-resistant Mycobacterium tuberculosis in Ganzhou, China.BMC infectious diseases · 2025Article
- Emergence of highly virulent multidrug and extensively drug resistant Escherichia coli and Klebsiella pneumoniae in buffalo subclinical mastitis cases.Scientific reports · 2025Article
- Simplified Co-extraction of total Nucleic Acids from Respiratory Samples for detection ofmedRxiv : the preprint server for health sciences · 2025Article
- Non-tuberculous mycobacteria and other acid fast bacilli pathogens identification by qPCR and MALDI-ToF MS in tuberculosis-like lesions of slaughtered cattle from Ecuador.Frontiers in veterinary science · 2025Article
- Comparative evaluation of MALDI-ToF mass spectrometry and Sanger sequencing of theFrontiers in cellular and infection microbiology · 2025Article
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- Rapid and accurate identification and differentiation ofFrontiers in public health · 2024Article
- The Role of Next-Generation Sequencing (NGS) in the Management of Tuberculosis: Practical Review for Implementation in Routine.Pathogens (Basel, Switzerland) · 2023Review
- Evaluation of DNA Extraction Methods for Reliable Quantification ofBiosensors · 2023Article
- Pulmonary non-tuberculous mycobacteria in colonisation and disease in The Gambia.Scientific reports · 2022Article
- Prediction of drug resistance profile of multidrug-resistant Mycobacterium tuberculosis (MDR-MTB) isolates from newly diagnosed case by whole genome sequencing (WGS): a study from a high tuberculosis burden country.BMC infectious diseases · 2022Article
- Antibiotic-resistant bacteria, antibiotic resistance genes, and antibiotic residues in wastewater from a poultry slaughterhouse after conventional and advanced treatments.Scientific reports · 2021Article
- High-Throughput Metagenomics for Identification of Pathogens in the Clinical Settings.Small methods · 2021Review
- A SIMPLE METHOD OF DNA EXTRACTION OFAfrican journal of infectious diseases · 2021Article
- Virulence factors analysis and antibiotic resistance of uropathogenicIranian journal of microbiology · 2020Article
- ESKAPE Bacteria and Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolated from Wastewater and Process Water from German Poultry Slaughterhouses.Applied and environmental microbiology · 2020Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Six methods of extracting Mycobacterium tuberculosis DNA from sputum for testing by quantitative PCR were compared: Tris-EDTA (TE) buffer, PrepMan Ultra, 2% sodium dodecyl sulfate (SDS)-10% Triton X with and without sonication, Infectio Diagnostics, Inc. (IDI) lysing tubes, and QIAGEN QIAamp DNA mini kit; all included a 15-min boiling step. Pooled digested and decontaminated sputum was spiked with M. tuberculosis ATCC 27294. Each extraction method was repeated eight times. Quantitative PCR was performed on the Smart Cycler and Rotor-Gene 3000 using primers targeting an 83-bp fragment of IS6110. An minor grove binding Eclipse probe with a fluorescent label was used for detection. An internal control was included to detect amplification inhibition. The limit of detection of M. tuberculosis DNA was 0.5 fg with both instruments. Calculated DNA concentrations (picograms) extracted using IDI, PrepMan, QIAGEN, and TE were 42.8, 30.4, 28.2, and 7.4, respectively, when run on the Smart Cycler, and 51.7, 20.1, 14.9, and 8.6, respectively, when run on Rotor-Gene. All extractions using SDS/Triton X with or without sonication were inhibited. Of the extraction methods evaluated, IDI lysis tubes provided the greatest yield of mycobacterial DNA, and the procedure can be completed in less than 1 h versus 2.5-3 h for the QIAGEN extraction.
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Registered trials
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