ArticleJournal of clinical microbiology2005
Loop-mediated isothermal amplification method for rapid detection of the periodontopathic bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Measuring the Invisible: Microbial Diagnostics for Periodontitis-A Narrative Review.International journal of molecular sciences · 2025Review
- The application of the loop-mediated isothermal amplification method for rapid detection of methicillin-resistantNew microbes and new infections · 2022Article
- Review
- The Loop-Mediated Isothermal Amplification Technique in Periodontal Diagnostics: A Systematic Review.Journal of clinical medicine · 2021Review
- Adjunctive Antiseptic Irrigation of Periodontal Pockets: Effects on Microbial and Cytokine Profiles.Dentistry journal · 2020Article
- The Development of Highly Specific and Sensitive Primers for the Detection of Potentially Allergenic Soybean (Foods (Basel, Switzerland) · 2020Article
- Rapid bacterial detection and antibiotic susceptibility testing in whole blood using one-step, high throughput blood digital PCR.Lab on a chip · 2020Article
- Article
- Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies.PLoS neglected tropical diseases · 2018Article
- Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications.Sensors and actuators. B, Chemical · 2018Review
- Development of a loop-mediated isothermal amplification method for the rapid diagnosis of Ascochyta rabiei L. in chickpeas.Scientific reports · 2016Article
- Three Tests Used to Identify Non-Culturable Form of Helicobacter pylori in Water Samples.Jundishapur journal of microbiology · 2015Article
- Development of Loop-Mediated Isothermal Amplification (LAMP) assay for rapid detection of Fusarium oxysporum f. sp. ciceris - wilt pathogen of chickpea.BMC research notes · 2015Article
- Application of loop-mediated isothermal amplification (LAMP) assay for the rapid diagnosis of pathogenic bacteria in clinical sputum specimens of acute exacerbation of COPD (AECOPD).International journal of clinical and experimental medicine · 2015Article
- Isothermal amplification methods for the detection of nucleic acids in microfluidic devices.Biosensors · 2013Review
- Development of a loop-mediated isothermal amplification assay for detection of Cronobacter spp. (Enterobacter sakazakii).World journal of microbiology & biotechnology · 2012Article
- The oral microflora in obesity and type-2 diabetes.Journal of oral microbiology · 2012Article
- Development of loop-mediated isothermal amplification (LAMP) assays for rapid detection of Ehrlichia ruminantium.BMC microbiology · 2010Article
- Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples.FEMS immunology and medical microbiology · 2010Article
- African trypanosomiasis: sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA.International journal for parasitology · 2008Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method, was developed for the rapid detection of the major periodontal pathogens Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. The LAMP method amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions using a set of four specially designed primers and a DNA polymerase with strand displacement activity. In this study, we initially designed the primers for LAMP assays to detect these bacteria and evaluated the specificity and sensitivity of these assays. The specificities of the primers for these bacteria were examined using various oral bacteria and various reaction times. The lower detection limits of the 60-min LAMP reaction without loop primers were 1 microg/tube for P. gingivalis, 10 fg/tube for T. forsythia, and 1 ng/tube for T. denticola. Addition of the loop primers for each bacterium improved the detection specificities and sensitivities by several magnitudes. Furthermore, LAMP assays were applied to the rapid detection of these periodontal pathogens in clinical specimens, and the results were compared with those of conventional PCR detection. The results of the LAMP assays corresponded to those of conventional PCR assays. These results indicate that the LAMP assay is an extremely rapid, highly sensitive, specific method. This method is very useful for the rapid detection of periodontopathic bacteria and the diagnosis of periodontal disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.