ArticleJournal of clinical microbiology2005
Multilocus sequence typing of Streptococcus uberis provides sensitive and epidemiologically relevant subtype information and reveals positive selection in the virulence gene pauA.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
- Detection of immunoreactive proteins ofFrontiers in cellular and infection microbiology · 2023Article
- Characterisation of Staphylococci Isolated from Milk Samples of a Water Buffalo Herd.Antibiotics (Basel, Switzerland) · 2022Article
- Multilocus Sequence Genotype Heterogeneity inAnimals : an open access journal from MDPI · 2022Article
- Occurrence of virulence-associated genes inVeterinarni medicina · 2022Article
- EnvironmentalAnimals : an open access journal from MDPI · 2021Article
- Sequence characterisation and novel insights into bovine mastitis-associated Streptococcus uberis in dairy herds.Scientific reports · 2021Article
- Molecular variability ofCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2019Article
- Identification and characterization of Staphylococcus devriesei isolates from bovine intramammary infections in KwaZulu-Natal, South Africa.BMC veterinary research · 2018Article
- Evidence of the genetic diversity and clonal population structure of Oenococcus oeni strains isolated from different wine-making regions of China.Journal of microbiology (Seoul, Korea) · 2018Article
- Application of a Dot Blot Hybridization Platform to AssessFrontiers in microbiology · 2017Article
- Molecular Epidemiology of Streptococcus uberis Clinical Mastitis in Dairy Herds: Strain Heterogeneity and Transmission.Journal of clinical microbiology · 2016Article
- Correlation of hypothetical virulence traits of two Streptococcus uberis strains with the clinical manifestation of bovine mastitis.Veterinary research · 2015Article
- Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis.BMC microbiology · 2012Article
- Functional bias of positively selected genes in Streptococcus genomes.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Article
- Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans.Journal of mammary gland biology and neoplasia · 2011Review
- Phylogenetic analysis of Clostridium botulinum type A by multi-locus sequence typing.Microbiology (Reading, England) · 2008Article
- Identification of Streptococcus uberis multilocus sequence types highly associated with mastitis.Applied and environmental microbiology · 2008Article
- Population genetic structure of the Staphylococcus intermedius group: insights into agr diversification and the emergence of methicillin-resistant strains.Journal of bacteriology · 2007Article
- Streptococcus mutans clonal variation revealed by multilocus sequence typing.Journal of clinical microbiology · 2007Article
- Extent of horizontal gene transfer in evolution of Streptococci of the salivarius group.Journal of bacteriology · 2007Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Control of the bovine mastitis pathogen Streptococcus uberis requires sensitive and epidemiologically meaningful subtyping methods that can provide insight into this pathogen's epidemiology and evolution. Development of a multilocus sequence typing (MLST) scheme based on six housekeeping and virulence genes allowed differentiation of 40 sequence types among 50 S. uberis isolates from the United States (n = 30) and The Netherlands (n = 20). MLST was more discriminatory than EcoRI or PvuII ribotyping and provided subtype data with better epidemiological relevance, e.g., by discriminating isolates with identical ribotypes obtained from different farms. Phylogenetic analyses of MLST data revealed indications of reticulate evolution between genes, preventing construction of a core phylogeny based on concatenated DNA sequences. However, all individual gene phylogenies clearly identified a distinct pauA-negative subtaxon of S. uberis for which housekeeping alleles closely resembled those of Streptococcus parauberis. While the average GC content for five genes characterized was between 0.38 and 0.40, pauA showed a considerably lower GC content (0.34), suggesting acquisition through horizontal transfer. pauA also showed a higher nonsynonymous/synonymous rate ratio (dN/dS) (1.2) compared to the other genes sequenced (dN/dS < 0.12), indicating positive selection in this virulence gene. In conclusion, our data show that (i) MLST provides for highly discriminatory and epidemiologically relevant subtyping of S. uberis; (ii) S. uberis has a recombinatorial population structure; (iii) phylogenetic analysis of MLST data reveals an S. uberis subtaxon resembling S. parauberis; and (iv) horizontal gene transfer and positive selection contribute to evolution of certain S. uberis genes, such as the virulence gene pauA.
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