ArticleJournal of clinical microbiology2005
Effect of sequence polymorphisms on performance of two real-time PCR assays for detection of herpes simplex virus.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Designing sensitive viral diagnostics with machine learning.Nature biotechnology · 2022Article
- Systematic evaluation of TaqMan real-time polymerase chain reaction assays targeting theVeterinary world · 2022Article
- In silico re-assessment of a diagnostic RT-qPCR assay for universal detection of Influenza A viruses.Scientific reports · 2019Article
- Improving the standardization of mRNA measurement by RT-qPCR.Biomolecular detection and quantification · 2018Article
- Hepatitis E Virus (HEV) Detection and Quantification by a Real-Time Reverse Transcription-PCR Assay Calibrated to the World Health Organization Standard for HEV RNA.Journal of clinical microbiology · 2017Article
- Evaluation of TaqMan qPCR System Integrating Two Identically Labelled Hydrolysis Probes in Single Assay.Scientific reports · 2017Article
- Preclinical Assessment of a Fully Automated Multiplex PCR Panel for Detection of Central Nervous System Pathogens.Journal of clinical microbiology · 2016Article
- Development and evaluation of the quantitative real-time PCR assay in detection and typing of herpes simplex virus in swab specimens from patients with genital herpes.International journal of clinical and experimental medicine · 2015Article
- Article
- tcdA As a diagnostic target in a loop-mediated amplification assay for detecting toxigenic Clostridium difficile.Journal of clinical laboratory analysis · 2013Article
- SNPs and real-time quantitative PCR method for constitutional allelic copy number determination, the VPREB1 marker case.BMC medical genetics · 2011Article
- Clinical course and spectrum of intensive care unit patients reactivating herpes simplex-1 virus: a retrospective analysis.Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine · 2008Article
- Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers.Journal of clinical microbiology · 2008Article
- Sequence polymorphism can produce serious artefacts in real-time PCR assays: hard lessons from Pacific oysters.BMC genomics · 2008Article
- Contrasting geographic distribution profiles of the herpes simplex virus type 1 BgOL and BgKL variants in Japan suggest dispersion and replacement.Journal of clinical microbiology · 2007Article
- A single-tube nucleic acid extraction, amplification, and detection method using aluminum oxide.The Journal of molecular diagnostics : JMD · 2006Article
- Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey.Virology journal · 2005Article
- Detection and monitoring of virus infections by real-time PCR.Molecular aspects of medicineReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Herpes simplex virus (HSV) is the most common cause of acquired, sporadic encephalitis in the United States. PCR identification of HSV in spinal fluid has become the diagnostic gold standard due to its sensitivity and potential for speed, replacing other methods such as culture. We developed a real-time PCR assay to detect HSV, using a new type of hybridization probe, the Eclipse probe. In this study, we ran 323 samples (171 positives and 152 negatives) with the Eclipse real-time PCR assay and compared these results with another PCR assay using gel detection. The real-time assay agreed with our reference method for 319 out of the 323 samples tested (99%). Using two different real-time PCR platforms, we discovered that SNPs within the amplicon's probe binding region that are used to distinguish HSV-1 from HSV-2 can decrease assay sensitivity. This problem is potentially a general one for assays using fluorescent probes to detect target amplification in a real-time format. While real-time PCR can be a powerful tool in the field of infectious disease, careful sequence evaluation and clinical validation are essential in creating an effective assay.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.