ArticleJournal of clinical microbiology2005
Comparison of genotypic and phenotypic methods for species-level identification of clinical isolates of coagulase-negative staphylococci.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
84 citing papers in PubMed, 1 synthesis or guideline pooled it, 249 citations in OpenAlex.
- Phoenix 100 versus Vitek 2 in the identification of gram-positive and gram-negative bacteria: a comprehensive meta-analysis.Journal of clinical microbiology · 2011Pooled it
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- Phylogenetic Footprints of Coagulase-Negative Staphylococci and Mammaliicoccus Isolated From Raw Caprine Milk.MicrobiologyOpen · 2025Article
- Scalable genotyping of microbial colonies.Microbial genomics · 2025Article
- The developing pig respiratory microbiome harbors strains antagonistic to common respiratory pathogens.mSystems · 2024Article
- Infective Endocarditis Caused by Staphylococcus lugdunensis.Internal medicine (Tokyo, Japan) · 2024Article
- Basic concepts, recent advances, and future perspectives in the diagnosis of bovine mastitis.Journal of veterinary science · 2024Review
- Current Limitations of Staph Infection Diagnostics, and the Role for VOCs in Achieving Culture-Independent Detection.Pathogens (Basel, Switzerland) · 2023Review
- Species identification by MALDI-TOF MS and gap PCR-RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and goat mastitis.Veterinary research · 2022Article
- Methicillin-Resistant Staphylococci (MRS): Carriage and Antibiotic Resistance Patterns in College Students.Journal of community health · 2022Article
- Effect of Processing Treatment and Modified Atmosphere Packing on Carrot's Microbial Community Structure by Illumina MiSeq Sequencing.Molecules (Basel, Switzerland) · 2022Article
- Genome-Based Characterization of a Plasmid-Associated Micrococcin P1 Biosynthetic Gene Cluster and Virulence Factors in Mammaliicoccus sciuri IMDO-S72.Applied and environmental microbiology · 2022Article
- Article
- Coagulase-Negative Staphylococci Clones Are Widely Distributed in the Hospital and Community.Pathogens (Basel, Switzerland) · 2021Article
- Comparative characterisation of human and ovine non-Epidemiology and infection · 2021Article
- Prevalence and antimicrobial drug resistance ofVeterinary world · 2020Article
- The Epidome - a species-specific approach to assess the population structure and heterogeneity of Staphylococcus epidermidis colonization and infection.BMC microbiology · 2020Article
- The Use of Less Conventional Meats or Meat with High pH Can Lead to the Growth of Undesirable Microorganisms during Natural Meat Fermentation.Foods (Basel, Switzerland) · 2020Article
- Performance and Application of 16S rRNA Gene Cycle Sequencing for Routine Identification of Bacteria in the Clinical Microbiology Laboratory.Clinical microbiology reviews · 2020Review
24 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To compare commonly used phenotypic methods with genotypic identification methods 47 clinical isolates of coagulase-negative staphylococci (CONS), 10 CONS ATCC strains, and a Staphylococcus aureus clinical isolate were identified using the API Staph ID test, BD Phoenix Automated Microbiology System, and 16S rRNA gene and tuf gene sequencing. When necessary part of the sodA gene was sequenced for definitive identification. The results show that tuf gene sequencing is the best method for identification of CONS, but the API Staph ID test is a reasonably reliable phenotypic alternative. The performance of the BD Phoenix Automated Microbiology System for identification of CONS is poor. The present study also showed that although genotypic methods are clearly superior to phenotypic identifications, a drawback of sequence-based genotypic methods may be a lack of quality of deposited sequences in data banks. In particular, 16S rRNA gene sequencing suffers from the lack of high quality among sequences deposited in GenBank. Furthermore, genotypic identification based on 16S rRNA sequences has limited discriminating power for closely related Staphylococcus species. We propose partial sequencing of the tuf gene as a reliable and reproducible method for identification of CONS species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.