ArticleJournal of clinical microbiology2005
Multilocus sequence typing lacks the discriminatory ability of pulsed-field gel electrophoresis for typing Salmonella enterica serovar Typhimurium.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 100 citations in OpenAlex.
- Shared Extended-Spectrum β-Lactamase-ProducingMicroorganisms · 2020Article
- Assessment and Comparison of Molecular Subtyping and Characterization Methods forFrontiers in microbiology · 2019Review
- Prevalence, Serotyping, Molecular Typing, and Antimicrobial Resistance ofFrontiers in microbiology · 2018Article
- Phenotypic and Genotypic Eligible Methods forFrontiers in microbiology · 2017Review
- Subtyping of Salmonella enterica Subspecies I Using Single-Nucleotide Polymorphisms in Adenylate Cyclase.Foodborne pathogens and disease · 2016Article
- Differential single nucleotide polymorphism-based analysis of an outbreak caused by Salmonella enterica serovar Manhattan reveals epidemiological details missed by standard pulsed-field gel electrophoresis.Journal of clinical microbiology · 2015Article
- Genome analysis and CRISPR typing of Salmonella enterica serovar Virchow.BMC genomics · 2014Article
- Whole-genome mapping: a new paradigm in strain-typing technology.Journal of clinical microbiology · 2013Review
- Molecular serotyping of Salmonella enterica by complete rpoB gene sequencing.Journal of microbiology (Seoul, Korea) · 2012Article
- Improving resolution of public health surveillance for human Salmonella enterica serovar Typhimurium infection: 3 years of prospective multiple-locus variable-number tandem-repeat analysis (MLVA).BMC infectious diseases · 2012Article
- Genetic relationships of phage types and single nucleotide polymorphism typing of Salmonella enterica Serovar Typhimurium.Journal of clinical microbiology · 2012Article
- Salmonella strains isolated from Galápagos iguanas show spatial structuring of serovar and genomic diversity.PloS one · 2012Article
- Methodologies for Salmonella enterica subsp. enterica subtyping: gold standards and alternatives.Applied and environmental microbiology · 2011Review
- Pulsed-field gel electrophoresis supports the presence of host-adapted Salmonella enterica subsp. enterica serovar Typhimurium strains in the British garden bird population.Applied and environmental microbiology · 2011Article
- Salmonella enterica in swine production: assessing the association between amplified fragment length polymorphism and epidemiological units of concern.Applied and environmental microbiology · 2011Article
- Molecular and comparative analysis of Salmonella enterica Senftenberg from humans and animals using PFGE, MLST and NARMS.BMC microbiology · 2011Article
- Novel virulence gene and clustered regularly interspaced short palindromic repeat (CRISPR) multilocus sequence typing scheme for subtyping of the major serovars of Salmonella enterica subsp. enterica.Applied and environmental microbiology · 2011Article
- Improved identification of epidemiologically related strains of Salmonella enterica by use of a fusion algorithm based on pulsed-field gel electrophoresis and multiple-locus variable-number tandem-repeat analysis.Journal of clinical microbiology · 2010Article
- Avian-pathogenic Escherichia coli strains are similar to neonatal meningitis E. coli strains and are able to cause meningitis in the rat model of human disease.Infection and immunity · 2010Article
- Pulsed-field gel electrophoresis diversity of human and bovine clinical Salmonella isolates.Foodborne pathogens and disease · 2010Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nontyphoidal salmonellae are among the leading causes of food-borne disease in the United States. Because of the importance of Salmonella enterica in food-borne disease, numerous typing methodologies have been developed. Among the several molecular typing methods, pulsed-field gel electrophoresis (PFGE) is currently considered the "gold standard" technique in typing Salmonella. The aim of this study was to compare the discriminatory power of PFGE to multilocus sequence typing (MLST) in typing Salmonella enterica serovar Typhimurium clinical isolates. A total of 85 Salmonella Typhimurium clinical isolates from cattle were used in this study. PFGE using XbaI was performed on the 85 isolates by the Centers for Disease Control and Prevention method, and data were analyzed using the BioNumerics software package. Fifty PFGE profiles were observed among the isolates, and these grouped into three major clusters. For the MLST analysis, the manB, pduF, glnA, and spaM genes were amplified by PCR from the same 85 isolates. DNA sequencing of these four genes, manB, pduF, glnA, and spaM, showed no genetic diversity among the isolates tested, with a 100% identity in nucleotide sequence. Moreover, the DNA sequences of the aforementioned genes showed 100% identity to the sequence reported in GenBank for the S. enterica serovar Typhimurium LT2 strain. Therefore, MLST, using these genes, lacks the discriminatory power of PFGE for typing Salmonella enterica serovar Typhimurium.
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