ArticleRNA (New York, N.Y.)2005
Characterization of conserved sequence elements in eukaryotic RNase P RNA reveals roles in holoenzyme assembly and tRNA processing.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Accumulation of noncoding RNA due to an RNase P defect in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2011Article
- The dual use of RNA aptamer sequences for affinity purification and localization studies of RNAs and RNA-protein complexes.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Pre-tRNA turnover catalyzed by the yeast nuclear RNase P holoenzyme is limited by product release.RNA (New York, N.Y.) · 2009Article
- Functional reconstitution and characterization of Pyrococcus furiosus RNase P.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Functional characterization of the conserved amino acids in Pop1p, the largest common protein subunit of yeast RNases P and MRP.RNA (New York, N.Y.) · 2006Article
- Ribonuclease P: the evolution of an ancient RNA enzyme.Critical reviews in biochemistry and molecular biologyArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
RNase P is a ubiquitous endoribonuclease responsible for cleavage of the 5' leader of precursor tRNAs (pre-tRNAs). Although the protein composition of RNase P holoenzymes varies significantly among Bacteria, Archaea, and Eukarya, the holoenzymes have essential RNA subunits with several sequences and structural features that are common to all three kingdoms of life. Additional structural elements of the RNA subunits have been found that are conserved in eukaryotes, but not in bacteria, and might have functions specifically required by the more complex eukaryotic holoenzymes. In this study, we have mutated four eukaryotic-specific conserved regions in Saccharomyces cerevisiae nuclear RNase P RNA and characterized the effects of the mutations on cell growth, enzyme function, and biogenesis of RNase P. RNase P with mutations in each of the four regions tested is sufficiently functional to support life although growth of the resulting yeast strains was compromised to varying extents. Further analysis revealed that mutations in three different regions cause differential defects in holoenzyme assembly, localization, and pre-tRNA processing in vivo and in vitro. These data suggest that most, but not all, eukaryotic-specific conserved regions of RNase P RNA are important for the maturation and function of the holoenzyme.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.