ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
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- GαJournal of neurochemistry · 2025Article
- Physiological Perspectives on Molecular Mechanisms and Regulation of Vesicular Glutamate Transport: Lessons From Calyx of Held Synapses.Frontiers in cellular neuroscience · 2021Review
- VGLUT1 functions as a glutamate/proton exchanger with chloride channel activity in hippocampal glutamatergic synapses.Nature communications · 2017Article
- Presynaptic Molecular Determinants of Quantal Size.Frontiers in synaptic neuroscience · 2016Review
- Drosophila melanogaster as a genetic model system to study neurotransmitter transporters.Neurochemistry international · 2014Review
- The α-subunit of the trimeric GTPase Go2 regulates axonal growth.Journal of neurochemistry · 2013Article
- Histaminylation of glutamine residues is a novel posttranslational modification implicated in G-protein signaling.FEBS letters · 2012Article
- Vesicular and plasma membrane transporters for neurotransmitters.Cold Spring Harbor perspectives in biology · 2012Review
- The Role of Inhibitory G Proteins and Regulators of G Protein Signaling in the in vivo Control of Heart Rate and Predisposition to Cardiac Arrhythmias.Frontiers in physiology · 2012Article
- Quantal amplitude at the cone ribbon synapse can be adjusted by changes in cytosolic glutamate.Molecular vision · 2011Article
- Coordinated control of sensitivity by two splice variants of Gα(o) in retinal ON bipolar cells.The Journal of general physiology · 2010Article
- Gαo represses insulin secretion by reducing vesicular docking in pancreatic beta-cells.Diabetes · 2010Article
- Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- A chloride conductance in VGLUT1 underlies maximal glutamate loading into synaptic vesicles.Nature neuroscience · 2009Article
- Synaptic vesicle protein trafficking at the glutamate synapse.Neuroscience · 2009Review
- Molecular mechanisms of go signaling.Neuro-Signals · 2009Review
- Deletion of Go2alpha abolishes cocaine-induced behavioral sensitization by disturbing the striatal dopamine system.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Classical neurotransmitters, including monoamines, acetylcholine, glutamate, GABA, and glycine, are loaded into synaptic vesicles by means of specific transporters. Vesicular monoamine transporters are under negative regulation by alpha subunits of trimeric G-proteins, including Galpha(o2) and Galpha(q). Furthermore, glutamate uptake, mediated by vesicular glutamate transporters (VGLUTs), is decreased by the nonhydrolysable GTP-analog guanylylimidodiphosphate. Using mutant mice lacking various Galpha subunits, including Galpha(o1), Galpha(o2), Galpha(q), and Galpha11, and a Galpha(o2)-specific monoclonal antibody, we now show that VGLUTs are exclusively regulated by Galpha(o2). G-protein activation does not affect the electrochemical proton gradient serving as driving force for neurotransmitter uptake; rather, Galpha(o2) exerts its action by specifically affecting the chloride dependence of VGLUTs. All VGLUTs show maximal activity at approximately 5 mm chloride. Activated Galpha(o2) shifts this maximum to lower chloride concentrations. In contrast, glutamate uptake by vesicles isolated from Galpha(o2-/-) mice have completely lost chloride activation. Thus, Galpha(o2) acts on a putative regulatory chloride binding domain that appears to modulate transport activity of vesicular glutamate transporters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.