ArticleMolecular biology of the cell2005
Structural and functional dissection of the Abp1 ADFH actin-binding domain reveals versatile in vivo adapter functions.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Dynamic remodeling of actin networks by cyclase-associated protein and CAP-Abp1 complexes.Current biology : CB · 2023Article
- Spatio-temporal regulation of endocytic protein assembly by SH3 domains in yeast.Molecular biology of the cell · 2023Article
- Wbm0076, a candidate effector protein of the Wolbachia endosymbiont of Brugia malayi, disrupts eukaryotic actin dynamics.PLoS pathogens · 2023Article
- Abp1 promotes Arp2/3 complex-dependent actin nucleation and stabilizes branch junctions by antagonizing GMF.Nature communications · 2018Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Drebrin contains a cryptic F-actin-bundling activity regulated by Cdk5 phosphorylation.The Journal of cell biology · 2013Article
- Drebrin-induced stabilization of actin filaments.The Journal of biological chemistry · 2013Article
- Inactivation of Tor proteins affects the dynamics of endocytic proteins in early stage of endocytosis.Journal of biosciences · 2013Article
- Article
- The importance of conserved features of yeast actin-binding protein 1 (Abp1p): the conditional nature of essentiality.Genetics · 2012Article
- Minimal requirements for actin filament disassembly revealed by structural analysis of malaria parasite actin-depolymerizing factor 1.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Conformational dynamics of actin: effectors and implications for biological function.Cytoskeleton (Hoboken, N.J.) · 2010Review
- NMR solution structures of actin depolymerizing factor homology domains.Protein science : a publication of the Protein Society · 2009Article
- Reconstitution and dissection of the 600-kDa Srv2/CAP complex: roles for oligomerization and cofilin-actin binding in driving actin turnover.The Journal of biological chemistry · 2009Article
- Structure of the actin-depolymerizing factor homology domain in complex with actin.The Journal of cell biology · 2008Article
- Structural basis and evolutionary origin of actin filament capping by twinfilin.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Abp1 is a multidomain protein that regulates the Arp2/3 complex and links proteins involved in endocytosis to the actin cytoskeleton. All of the proposed cellular functions of Abp1 involve actin filament binding, yet the actin binding site(s) on Abp1 have not been identified, nor has the importance of actin binding for Abp1 localization and function in vivo been tested. Here, we report the crystal structure of the Saccharomyces cerevisiae Abp1 actin-binding actin depolymerizing factor homology (ADFH) domain and dissect its activities by mutagenesis. Abp1-ADFH domain and ADF/cofilin structures are similar, and they use conserved surfaces to bind actin; however, there are also key differences that help explain their differential effects on actin dynamics. Using point mutations, we demonstrate that actin binding is required for localization of Abp1 in vivo, the lethality caused by Abp1 overexpression, and the ability of Abp1 to activate Arp2/3 complex. Furthermore, we genetically uncouple ABP1 functions that overlap with SAC6, SLA1, and SLA2, showing they require distinct combinations of activities and interactions. Together, our data provide the first structural and functional view of the Abp1-actin interaction and show that Abp1 has distinct cellular roles as an adapter, linking different sets of ligands for each function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.