ArticleNucleic acids research2005
The catalytic mechanism of hairpin ribozyme studied by hydrostatic pressure.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Pressure pushes tRNAProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Origin of Life: What Is the Question?Astrobiology · 2022Article
- Hidden intermediates in Mango III RNA aptamer folding revealed by pressure perturbation.Biophysical journal · 2022Article
- Article
- Nucleic Acid Catalysis under Potential Prebiotic Conditions.Chemistry, an Asian journal · 2020Review
- Mechanochemistry of nucleosides, nucleotides and related materials.Beilstein journal of organic chemistry · 2018Review
- Pressure modulates the self-cleavage step of the hairpin ribozyme.Nature communications · 2017Article
- A Hypothesis: Life Initiated from Two Genes, as Deduced from the RNA World Hypothesis and the Characteristics of Life-Like Systems.Life (Basel, Switzerland) · 2016Article
- Hammerhead ribozyme activity and oligonucleotide duplex stability in mixed solutions of water and organic compounds.FEBS open bio · 2014Article
- Hydrostatic and osmotic pressure study of the RNA hydration.Molecular biology reports · 2012Article
- Replication of avocado sunblotch viroid in the yeast Saccharomyces cerevisiae.Journal of virology · 2011Article
- Effect of high hydrostatic pressure on hydration and activity of ribozymes.Molecular biology reports · 2010Article
- Efficient inhibition of hepatitis B virus replication by hepatitis delta virus ribozymes delivered by targeting retrovirus.Virology journal · 2010Article
- Construction of retroviral vector of p(125FAK) specific ribozyme genes and its effects on BGC-823 cells.World journal of gastroenterology · 2006Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The discovery of ribozymes strengthened the RNA world hypothesis, which assumes that these precursors of modern life both stored information and acted as catalysts. For the first time among extensive studies on ribozymes, we have investigated the influence of hydrostatic pressure on the hairpin ribozyme catalytic activity. High pressures are of interest when studying life under extreme conditions and may help to understand the behavior of macromolecules at the origins of life. Kinetic studies of the hairpin ribozyme self-cleavage were performed under high hydrostatic pressure. The activation volume of the reaction (34 +/- 5 ml/mol) calculated from these experiments is of the same order of magnitude as those of common protein enzymes, and reflects an important compaction of the RNA molecule during catalysis, associated to a water release. Kinetic studies were also carried out under osmotic pressure and confirmed this interpretation and the involvement of water movements (78 +/- 4 water molecules per RNA molecule). Taken together, these results are consistent with structural studies indicating that loops A and B of the ribozyme come into close contact during the formation of the transition state. While validating baro-biochemistry as an efficient tool for investigating dynamics at work during RNA catalysis, these results provide a complementary view of ribozyme catalytic mechanisms.
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