ArticleApplied and environmental microbiology2005
Construction of a ColD cda promoter-based SOS-green fluorescent protein whole-cell biosensor with higher sensitivity toward genotoxic compounds than constructs based on recA, umuDC, or sulA promoters.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis Driven Strain Design for Mitigating Oxidative Stresses Important in Biomanufacturing.ACS synthetic biology · 2024Pooled it
- Rapid detection of genotoxicity employing an engineered high-sensitivity and tight SOS-response biosensor inSynthetic and systems biotechnology · 2026Article
- Harnessing desiccation-tolerant cyanobacteria as biosensors for LexA-mediated responses in deep space.Frontiers in microbiology · 2026Article
- Antibacterial compounds against non-growing and intracellular bacteria.npj antimicrobials and resistance · 2025Article
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- Qualitative and quantitative assessment of genotoxins usingRSC advances · 2019Article
- A Combination of Linalool, Vitamin C, and Copper Synergistically Triggers Reactive Oxygen Species and DNA Damage and InhibitsApplied and environmental microbiology · 2019Article
- Design of synthetic epigenetic circuits featuring memory effects and reversible switching based on DNA methylation.Nature communications · 2017Article
- Bet-hedging in bacteriocin producing Escherichia coli populations: the single cell perspective.Scientific reports · 2017Article
- A novel high-throughput cell-based assay aimed at identifying inhibitors of DNA metabolism in bacteria.Antimicrobial agents and chemotherapy · 2014Article
- Detection of induced synthesis of colicin E9 using ColE9p::gfpmut2 based reporter system.World journal of microbiology & biotechnology · 2014Article
- ParA encoded on chromosome II of Deinococcus radiodurans binds to nucleoid and inhibits cell division in Escherichia coli.Journal of biosciences · 2013Article
- Transcription termination controls prophage maintenance in Escherichia coli genomes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Genetically modified whole-cell bioreporters for environmental assessment.Ecological indicators · 2013Article
- Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors.Antimicrobial agents and chemotherapy · 2012Article
- Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins.Applied and environmental microbiology · 2011Article
- Identification of Vibrio natriegens uvrA and uvrB genes and analysis of gene regulation using transcriptional reporter plasmids.Journal of microbiology (Seoul, Korea) · 2010Article
- Bacterial genotoxicity bioreporters.Microbial biotechnology · 2010Review
- Where microbiology meets microengineering: design and applications of reporter bacteria.Nature reviews. Microbiology · 2010Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Four different green fluorescent protein (GFP)-based whole-cell biosensors were created based on the DNA damage inducible SOS response of Escherichia coli in order to evaluate the sensitivity of individual SOS promoters toward genotoxic substances. Treatment with the known carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) revealed that the promoter for the ColD plasmid-borne cda gene had responses 12, 5, and 3 times greater than the recA, sulA, and umuDC promoters, respectively, and also considerably higher sensitivity. Furthermore, we showed that when the SOS-GFP construct was introduced into an E. coli host deficient in the tolC gene, the minimal detection limits toward mitomycin C, MNNG, nalidixic acid, and formaldehyde were lowered to 9.1 nM, 0.16 microM, 1.1 microM, and 141 microM, respectively, which were two to six times lower than those in the wild-type strain. This study thus presents a new SOS-GFP whole-cell biosensor which is not only able to detect minute levels of genotoxins but, due to its use of the green fluorescent protein, also a reporter system which should be applicable in high-throughput screening assays as well as a wide variety of in situ detection studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.